基于Grüneisen弛豫非线性光声效应的单波长浓度解析方法  

Concentration Quantification Method Based on Single-Wavelength Excitation via Grüneisen-Relaxation Nonlinear Photoacoustic Effect

作　　者：王夏天 吕圣苗 解志华 陈敬钦 龚小竞[2] 董立泉 Wang Xiatian;LüShengmiao;Xie Zhihua;Chen Jingqin;Gong Xiaojing;Dong Liquan(Beijing Key Laboratory for Precision Optoelectronic Measurement Instrument and Technology,School of Optics and Photonics,Beijing Institute of Technology,Beijing 100081,China;Research Center for Biomedical Optics and Molecular Imaging,Shenzhen Key Laboratory for Molecular Imaging,Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology,CAS Key Laboratory of Health Informatics,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,Guangdong,China;Yangtze Delta Region Academy of Beijing Institute of Technology(Jiaxing),Jiaxing 314019,Zhejiang,China)

机构地区：[1]北京理工大学光电学院精密光电测试仪器及技术北京市重点实验室,北京100081 [2]中国科学院深圳先进技术研究院生物医学光学与分子影像研究中心,深圳市分子影像学重点实验室,广东省生物医学光学影像技术重点实验室,中国科学院健康信息学重点实验室,广东深圳518055 [3]北京理工大学长三角研究院(嘉兴),浙江嘉兴314019

出　　处：《光学学报》2023年第23期216-224,共9页Acta Optica Sinica

基　　金：国家自然科学基金(61975226,61705250,82027803);中国科学院健康信息学重点实验室(2011DP173015);广东省自然科学基金(2021A1515011949);广东省生物医学光学成像技术重点实验室(2021A1515011949);深圳市科技创新委员会(JCYJ20220818101417039,ZDSY20130401165820357)。

摘　　要：光声分子影像技术在生物医学中具有广泛的应用。实现分子探针浓度的准确解析,对于相关疾病的研究具有重要意义。然而,活体探测时,来源于生物组织的信号与探针的信号混叠,增加了准确解析的难度。当前改善的方法:或需要使用多波长进行探测,导致解析速度慢而且过程复杂;或使用单波长的方法,但需要依托于一类特殊探针的开关功能,导致普适性不足。鉴于此,提出了一种基于Grüneisen弛豫非线性光声效应的单波长浓度解析方法,并分别对色素染料与罗丹明6G探针两类样品开展了解析实验。结果表明,本文提出的方法获得的解析结果比线性单波长方法的解析结果误差更小,并且具有较好的普适性,为光声分子影像的浓度解析方法提供了新的思路。Objective Photoacoustic molecular imaging has been extensively applied to biomedical research.The accurate quantification of molecular probe concentrations has paramount importance in relevant disease investigation.Nevertheless,during in-vivo detection,the signals emitted by exogenous probes are often mixed with those originating from endogenous biological tissues,thereby diminishing the quantification accuracy of probe concentrations.Traditional photoacoustic molecular imaging relies on the linear photoacoustic effect,and some methods have been proposed to enhance the concentration quantification accuracy under complex environments.One approach is based on multi-wavelength detection,but multi-wavelength switching complicates the signal acquisition process and reduces the quantification speed.Another approach is proposed according to single-wavelength background subtraction,while it lacks universality due to reliance on employing molecular probes with switchable responses.Consequently,it becomes an urgent need to develop a concentration quantification method predicated on single-wavelength excitation that does not depend on specific probe responses to advance photoacoustic molecular imaging.The Grüneisen-relaxation nonlinear photoacoustic effect exhibits promising potential to meet this need.Unlike the linear relationship between signal amplitude and absorption coefficient in the linear photoacoustic effect,the Grüneisen-relaxation(GR)photoacoustic effect shows a quadratic nonlinearity between the two physical parameters.Based on this,we propose a method for quantifying concentrations using the Grüneisen-relaxation nonlinear photoacoustic effect by single-wavelength excitation. This approach adopts the nonlinear relationship between the two physical parameters to improve the ratio between the target and background signals, effectively diminishing the background interference. Consequently, we present a novel and promising solution for improving the concentration quantification accuracy.Methods To demonstrate th

关 键 词：生物技术 光声效应 Grüneisen弛豫 浓度解析 分子影像 

分 类 号：Q631[生物学—生物物理学]