浮游藻类叶绿素荧光产量与光合活性关系实验研究  

Experimental Study on Relationship Between Chlorophyll Fluorescence Yield and Photosynthetic Activity in Planktonic Algae

作　　者：刘津京 殷高方 赵南京[1,2,3] 张小玲[4] 甘婷婷 陈敏 董鸣 王勰 石高勇[1,2,3] 程钊 Liu Jinjing;Yin Gaofang;Zhao Nanjing;Zhang Xiaoling;Gan Tingting;Chen Min;Dong Ming;Wang Xie;Shi Gaoyong;Cheng Zhao(Key Laboratory of Environmental Optics and Technology,Chinese Academy of Sciences,Anhui Institute of Optics and Fine Mechanics,Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei 230031,Anhui,China;University of Science and Technology of China,Hefei 230026,Anhui,China;Key Laboratory of Optical Monitoring Technology for Environment of Anhui Province,Hefei 230031,Anhui,China;Institues of Physical Science and Information Technology,Anhui University,Hefei 230601,Anhui,China)

机构地区：[1]中国科学院合肥物质科学研究院安徽光学精密机械研究所中国科学院环境光学与技术重点实验室,安徽合肥230031 [2]中国科学技术大学,安徽合肥230026 [3]安徽省环境光学监测技术重点实验室,安徽合肥230031 [4]安徽大学物质科学与信息技术研究院,安徽合肥230601

出　　处：《光学学报》2023年第23期280-285,共6页Acta Optica Sinica

基　　金：国家重点研发计划(2022YFC3103901,2021YFC3200100);国家自然科学基金(62005001);安徽省科技重大专项(202003a07020007,202203a07020002)。

摘　　要：以蛋白核小球藻为研究对象,通过毒性胁迫、光照胁迫和温度改变蛋白核小球藻的光合活性,研究蛋白核小球藻叶绿素荧光产量与光合活性参数F_(v)/F_(m)的变化关系。结果表明:3种不同生长环境下,蛋白核小球藻的叶绿素荧光产量随着F_v/F_m改变而发生较为明显变化,最大变化范围为235~668(μg·L^(-1))^(-1);F_(v)/F_(m)与叶绿素荧光产量之间具有明显负线性相关性,线性优度R~2超过0.91。该研究结果为发展更为准确的藻类叶绿素a质量浓度活体荧光检测方法提供了重要依据。Objective We aim to study the influence of photosynthetic activity change on chlorophyll fluorescence yield and explore the error source of measuring algae chlorophyll a concentration by living fluorescence method,so as to provide an important basis for the subsequent development of on-site accurate detection method of algae chlorophyll concentration in water based on living fluorescence method.Methods We use ACT2&FastOcean FRRF algal fluorescence meter(CTG Company,UK)to measure the photosynthetic activity parameter F_(v)/F_(m).After 15 min dark adaptation of the algal sample(the photosynthetic reaction center of the algal sample is fully open),we selecte an excitation light source of 450 nm(the characteristic excitation wavelength of Chlorella pyrenoidosa)and measure the chlorophyll fluorescence induction curve.According to the basic parameters of the curve,the maximum photochemical quantum yield F_(v)/F_(m) is calculated.We use HJ897-2017 water quality determination of chlorophyll a spectrophotometry to measure the concentration of chlorophyll a of sample algae.In the experiment,the Hitach7000 fluorescence spectrophotometer is used to measure the three-dimensional fluorescence spectrum of Chlorella pyrenoidosa,as shown in Fig.1(a).The characteristic fluorescence spectrum region of Chlorella pyrenoidosa(excitation wavelength range of 380-500 nm and emission wavelength range of 660-750 nm)is selected,and the fluorescence intensity of living algae is obtained by fluorescence region integration method.The fluorescence yield,namely the fluorescence intensity per unit of chlorophyll a,is further obtained.Three groups of parallel samples are allocated for the experiment,and the final test results are taken as the average value,so as to study the change rule between photosynthetic activity and fluorescence yield of Chlorella pyrenoidosa.Results and Discussions Under the DCMU toxicity stress,the chlorophyll fluorescence yield and photosynthetic activity of Chlorella pyrenoidosa with different mass concentrations have th

关 键 词：光谱学 浮游藻类 活体荧光法 叶绿素荧光产量 光合活性 浓度检测 

分 类 号：Q914.82[天文地球—古生物学与地层学]