Adhesive cryogel particles for bridging confined and irregular tissue defects  

作　　者：Yao-Ting Xue Ming-Yu Chen Jia-Sheng Cao Lei Wang Jia-Hao Hu Si-Yang Li Ji-Liang Shen Xin-Ge Li Kai-Hang Zhang Shu-Qiang Hao Sarun Juengpanich Si-Bo Cheng Tuck-Whye Wong Xu-Xu Yang Tie-Feng Li Xiu-Jun Cai Wei Yang 

机构地区：[1]Department of Engineering Mechanics,Zhejiang University,Hang‑zhou 310027,China [2]Key Laboratory of Soft Machines and Smart Devices of Zhejiang Province,Zhejiang University,Hangzhou 310027,China [3]Center for X‑Mechanics,Department of Engineering Mechanics,Zhejiang Univer‑sity,Hangzhou 310027,China [4]Department of General Surgery,Sir Run-Run Shaw Hospital,Zhejiang University,Hangzhou 310016,China [5]Soft Intelligent Materials Co.,Ltd,Suzhou 215123,China [6]School of Biomedical Engineering and Health Sciences and Advanced Membrane Technology Research Centre,Universiti Teknologi Malaysia,81310 Skudai,Malaysia

出　　处：《Military Medical Research》2023年第6期763-777,共15页军事医学研究（英文版）

基　　金：supported by the National Natural Science Foundation of China (12102388, T2125009, 92048302);the National Key Research and Development Program of China 2017 (YFA0701100);the Fundamental Research Funds for the Central Universities (226-2022-00141, 2022QZJH52)。

摘　　要：Background Reconstruction of damaged tissues requires both surface hemostasis and tissue bridging.Tissues with damage resulting from physical trauma or surgical treatments may have arbitrary surface topographies,making tissue bridging challenging.Methods This study proposes a tissue adhesive in the form of adhesive cryogel particles(ACPs) made from chitosan,acrylic acid,1-ethyl-3-(3-dimethylaminopropyl) carbodiimide(EDC) and N-hydroxysuccinimide(NHS).The adhesion performance was examined by the 180-degree peel test to a collection of tissues including porcine heart,intestine,liver,muscle,and stomach.Cytotoxicity of ACPs was evaluated by cell proliferation of human normal liver cells(LO2)and human intestinal epithelial cells(Caco-2).The degree of inflammation and biodegradability were examined in dorsal subcutaneous rat models.The ability of ACPs to bridge irregular tissue defects was assessed using porcine heart,liver,and kidney as the ex vivo models.Furthermore,a model of repairing liver rupture in rats and an intestinal anastomosis in rabbits were established to verify the effectiveness,biocompatibility,and applicability in clinical surgery.Results ACPs are applicable to confined and irregular tissue defects,such as deep herringbone grooves in the parenchyma organs and annular sections in the cavernous organs.ACPs formed tough adhesion between tissues[(670.9±50.1) J/m^(2) for the heart,(607.6±30.0) J/m^(2) for the intestine,(473.7±37.0) J/m^(2) for the liver,(186.1±13.3) J/m^(2) for the muscle,and(579.3±32.3) J/m^(2) for the stomach].ACPs showed considerable cytocompatibility in vitro study,with a high level of cell viability for 3 d[(98.8±1.2)%for LO2 and(98.3±1.6)%for Caco-2].It has comparable inflammation repair in a ruptured rat liver(P=0.58 compared with suture closure),the same with intestinal anastomosis in rabbits(P=0.40 compared with suture anastomosis).Additionally,ACP-based intestinal anastomosis(less than 30 s) was remarkably faster than the conventional suturing process(more than 10 min).Whe

关 键 词：Tissue reconstruction Wet adhesion Adhesive hydrogel BIOADHESIVE 

分 类 号：R318.08[医药卫生—生物医学工程]