A tumor-associated heparan sulfate-related glycosaminoglycan promotes the generation of functional regulatory T cells  

作　　者：Leticia Martín-Cruz Marcos Viñuela Ioanna Kalograiaki Alba Angelina Paola Oquist-Phillips Irene Real-Arévalo Francisco Javier Cañada JoséIgnacio Tudela Luis Moltó Jesús Moreno-Sierra JoséLuis Subiza Oscar Palomares 

机构地区：[1]Department of Biochemistry and Molecular Biology,School of Chemistry,Complutense University of Madrid,Madrid,Spain [2]Inmunotek,Alcaláde Henares,Madrid,Spain [3]Fundación Investigación Hospital Clínico San Carlos,Martin Lagos s/n,28040,Madrid,Spain [4]Centro de Investigaciones Biológicas Margarita Salas,CSIC,Ramiro de Maetzu 9,28040,Madrid,Spain [5]CIBER de Enfermedades Respiratorias(CIBERES)Avda,Monforte de Lemos,3-5,28029,Madrid,Spain [6]Servicio de Urología,Hospital Clínico San Carlos,Universidad Complutense de Madrid,Martín Lagos s/n,28040,Madrid,Spain

出　　处：《Cellular & Molecular Immunology》2023年第12期1499-1512,共14页中国免疫学杂志（英文版）

基　　金：upported by grants FEI 16_60 from Art.834154605(138/2012)(24/2013),SAF-2017-84978-R from MINECO(Spain)and PID2020-114396RB-I00 from Ministerio de Ciencia e Innovación(Spain)to OP and PID2021-123781OB-C22 to FJC funded by MCIN/AEI/10.13039/501100011033(Spain)and RTC-2015-3805-1 from MINECO to Inmunotek SL.LM-C and AA were recipients of FPU and UCM predoctoral fellowships,respectively.

摘　　要：Functional Tregs play a key role in tumor development and progression,representing a major barrier to anticancer immunity.The mechanisms by which Tregs are generated in cancer and the influence of the tumor microenvironment on these processes remain incompletely understood.Herein,by using NMR,chemoenzymatic structural assays and a plethora of in vitro and in vivo functional analyses,we demonstrate that the tumoral carbohydrate A10(Ca10),a cell-surface carbohydrate derived from Ehrlich’s tumor(ET)cells,is a heparan sulfate-related proteoglycan that enhances glycolysis and promotes the development of tolerogenic features in human DCs.Ca10-stimulated human DCs generate highly suppressive Tregs by mechanisms partially dependent on metabolic reprogramming,PD-L1,IL-10,and IDO.Ca10 also reprograms the differentiation of human monocytes into DCs with tolerogenic features.In solid ET-bearing mice,we found positive correlations between Ca10 serum levels,tumor size and splenic Treg numbers.Administration of isolated Ca10 also increases the proportion of splenic Tregs in tumor-free mice.Remarkably,we provide evidence supporting the presence of a circulating human Ca10 counterpart(Ca10H)and show,for the first time,that serum levels of Ca10H are increased in patients suffering from different cancer types compared to healthy individuals.Of note,these levels are higher in prostate cancer patients with bone metastases than in prostate cancer patients without metastases.Collectively,we reveal novel molecular mechanisms by which heparan sulfate-related structures associated with tumor cells promote the generation of functional Tregs in cancer.The discovery of this novel structural-functional relationship may open new avenues of research with important clinical implications in cancer treatment.

关 键 词：Tumoral carbohydrate A10 Heparan sulfate-related proteoglycan Cancer immunology Dendritic cell Regulatory T cell 

分 类 号：R392.12[医药卫生—免疫学]