饮用出厂水中86种农药的固相萃取-超高效液相色谱-串联质谱同时测定法  

Simultaneous determination of 86 pesticides in drinking factory water by solid phase extraction ultra high performance liquid chromatography tandem mass spectrometry

作　　者：陶晶[1] 李婷 王春梅[1] 张桂斌[1] 张锐[1] 张永[1] TAO Jing;LI Ting;WANG Chunmei;ZHANG Guibin;ZHANG Rui;ZHANG Yong(Department of Environmental Health,Beijing Center for Disease Control and Prevention,Beijing,100013,China)

机构地区：[1]北京市疾病预防控制中心环境卫生所,北京100013

出　　处：《职业与健康》2023年第23期3174-3179,共6页Occupation and Health

基　　金：首都卫生发展科研专项(首发2020-2-3013)。

摘　　要：目的 建立固相萃取(solid phase extraction,SPE)-超高效液相色谱-串联质谱(ultra performance liquid chromatographytandem mass spectrometry,UPLC-MS/MS)测定饮用出厂水中86种农药的方法。方法 依据国内外饮水卫生标准,结合我国农药使用情况及检测方法适用性,选择86种农药为目标分析物,优化水样预处理方法及各农药的多离子反应监测(multiple reaction monitoring,MRM)条件进行方法评价,并应用于本地出厂水中农药残留检测。结果 86种农药在0.5~100μg/L范围内线性关系良好,相关系数为0.991 3~0.999 7,方法检出限为0.1~5.0 ng/L。其中36种农药的方法定量限(limit of quantitation,LOQ)为1 ng/L,29种为2 ng/L,21种为5 ng/L。86种农药在其LOQ及以上各加标水平下的回收率为59.5%~116.1%,相对标准偏差为1.6%~40.1%。结论 该方法准确、灵敏,经实际样品测试适用于生活饮用水中多种类农药残留的同时检测。Objective To establish a method for the determination of 86 pesticides in drinking factory water by solid phase extraction(SPE)-ultra high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS). Methods According to the domestic and foreign drinking water sanitation standards,combined with the use of pesticides in China and the applicability of detection methods,86 pesticides were selected as the target analytes. The water sample pretreatment method and the multi-ion reaction monitoring(MRM) conditions of pesticides were optimized for method evaluation,and applied to the detection of pesticide residues in local factory water. Results The linear relationship of 86 pesticides within the range of 0.5-100 μg/L was good,with a correlation coefficient of 0.991 3-0.999 7,and a detection limit of 0.1-5.0 ng/L. The limit of quantification(LOQ) of 36 pesticides was 1 ng/L,29 pesticides was 2 ng/L,and 21 pesticides was 5 ng/L. The recovery rates of 86 pesticides at LOQ and above spiking levels were 59.5%-116.1%,and the relative standard deviations were 1.6%-40.1%. Conclusion This method is accurate and sensitive,and is suitable for the simultaneous detection of multiple types of pesticide residues in drinking water after actual sample testing. Download(CAJ format) Download(PDF format)

关 键 词：固相萃取 超高效液相色谱-串联质谱 农药 生活饮用水 出厂水 

分 类 号：R115[医药卫生—公共卫生与预防医学]