济麦44/济麦229重组自交系群体籽粒蛋白质含量QTL分析  

QTL Analysis of Grain Protein Content in Recombinant Inbred Lines Derived from JM 44/JM 229

作　　者：单宝雪 刘秀坤 肖延军 展晓孟 黄金鑫 刘百川 张玉梅[1] 李豪圣[2] 刘建军[2] 高欣 曹新有[2] 赵振东[2] Shan Baoxue;Liu Xiukun;Xiao Yanjun;Zhan Xiaomeng;Huang Jinxin;Liu Baichuan;Zhang Yumei;Li Haosheng;Liu Jianjun;Gao Xin;Cao Xinyou;Zhao Zhendong(College of Agronomy,Qingdao Agricultural University,Qingdao 266109,China;Crop Research Institute,Shandong Academy of Agricultural Sciences/National Engineering Research Center for Wheat&Maize/Key Laboratory of Wheat Biology and Genetic Improvement in North Yellow&Huai River Valley,Ministry of Agriculture/Shandong Provincial Technology Innovation Center for Wheat,Jinan 250100,China)

机构地区：[1]青岛农业大学农学院,山东青岛266109 [2]山东省农业科学院作物研究所/小麦玉米国家工程中心/农业部黄淮北部小麦生物学与遗传育种重点实验室/山东省小麦技术创新中心,山东济南250100

出　　处：《山东农业科学》2024年第1期1-9,共9页Shandong Agricultural Sciences

基　　金：山东省自然科学基金项目(ZR202111220361);国家小麦产业技术体系项目(CARS-03-06);国家重点研发计划项目(2022YFD1200200)。

摘　　要：小麦籽粒蛋白质含量与面团流变学特性及加工特性的关系密不可分。本研究在2020—2021年济南试验基地(E1)及2021—2022年济南试验基地(E2)和济阳试验基地(E3)三个环境下,以“济麦44×济麦229”构建的包含285个家系的重组自交系群体(F2∶6RILs)为材料,利用小麦55K SNP芯片构建高密度遗传连锁图谱,对籽粒蛋白质含量进行QTL分析。结果共筛选到2344个SNP标记用于构建遗传连锁图谱,图谱总长度3349.95 cM,平均标记密度为1.43 cM/标记。通过对籽粒蛋白质含量的QTL分析,共检测到18个籽粒蛋白质含量相关QTL,分布在1A、1B、2B、3D、4B、4D、5A、5B、5D、7A、7B共11条染色体上。Qpc.saas.4B-1在E1、E2和E3三个环境和BLUE(最佳线性无偏估计)值中均被稳定检测到,可以解释3.26%~23.79%的表型变异。Qpc.saas.4D和Qpc.saas.5A在两个环境和BLUE值中被检测到,分别解释2.42%~11.18%和2.48%~5.47%的表型变异,且Qpc.saas.4D与小麦矮秆基因Rht-D1b物理位置重合。本研究中检测到的QTL新位点Qpc.saas.4B-1、Qpc.saas.4D和Qpc.saas.5A是控制籽粒蛋白质含量的主效基因,具有高表型变异解释率。本研究结果可为小麦品质育种提供分子标记及理论参考。The protein content of wheat grain is closely related to the rheological and processing properties of dough.In this study,under three enviroments of Jinan test base in 2020-2021(E1),Jinan(E2)and Jiyang(E3)test bases in 2021-2022,the wheat 55K SNP array was used to construct a high-density genetic linkage map of the recombinant inbred lines derived from‘JM 44×JM 229’(285,F2∶6 RILs)for QTL analysis of grain protein content.A total of 2344 SNP markers were used to construct the genetic linkage map with a total length of 3349.95 cM,and the average density was 1.43 cM per marker.Through QTL analysis,18 QTLs concerning grain protein content were detected,which distributed on 11 chromosomes:1A,1B,2B,3D,4B,4D,5A,5B,5D,7A and 7B.Qpc.saas.4B-1 was stably detected in all three environments and BLUE value,explaining 3.26%~23.79%of phenotypic variation.Qpc.saas.4D and Qpc.saas.5A were detected in two envi-ronments and BLUE value,explaining 2.42%~11.18%and 2.48%~5.47%of phenotypic variation,respec-tively.The physical location of Qpc.saas.4D coincides with that of wheat dwarf gene Rht-D1b.The new QTL lo-ci Qpc.saas.4B-1,Qpc.saas.4D and Qpc.saas.5A obtained in this study were major genes controlling grain pro-tein content,which had high explanation rates of phenotypic variation.The results above could provide molec-ular markers and theoretical references for quality breeding of wheat.

关 键 词：小麦 籽粒蛋白质含量 QTL分析 重组自交系 55K SNP芯片 

分 类 号：S512.110.3[农业科学—作物学]