沙门菌主要血清型标准菌株的筛选鉴定  

Identification of dominant serotypes of standard Salmonella strains

作　　者：高翔[1] 张龙飞 侯心娇 张萍[1] 甄博珺[1] 郭晓晨 孙慧颖 闫梅英[2] Gao Xiang;Zhang Longfei;Hou Xinjiao;Zhang Ping;Zhen Bojun;Guo Xiaochen;Sun Huiying;Yan Meiying(Tongzhou District Center for Disease Control and Prevention,Beijing 101100,China;State Key Laboratory for Intelligent Tracking and Forecasting Infectious Diseases,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]北京市通州区疾病预防控制中心,北京101100 [2]中国疾病预防控制中心传染病预防控制所,传染病溯源预警与智能决策国家重点实验室,北京102206

出　　处：《疾病监测》2023年第12期1446-1452,共7页Disease Surveillance

基　　金：国家科技重大专项(No.2018ZX10101002)。

摘　　要：目的依据《病原微生物菌(毒)种国家标准株评价技术标准》(WS/T 812—2022),获得8种沙门菌主要血清型标准株,并建立标准株筛选主要流程。方法分别对原代及传代沙门菌菌株进行形态学观察、生化试验及基因组测序分析,获得形态及生化典型、基因组稳定的标准菌株。结果从24株沙门菌中筛选获得8株分属8种血清型(伤寒沙门菌、甲型副伤寒沙门菌、乙型副伤寒沙门菌、丙型副伤寒沙门菌、鼠伤寒沙门菌、肠炎沙门菌、单相鼠伤寒沙门菌、猪霍乱沙门菌)的菌株,这8株菌在沙门菌推荐用选择性培养基上形态典型,生化反应典型,传代后基因组变异较小,血清凝集结果稳定,可以作为沙门菌检验的参比菌株。结论本研究通过筛选获得具有相应血清型病原特征的8种沙门菌主要血清型标准株,可作为沙门菌检测、血清分型质控、基因组进化分析的参比菌株,并为后续核酸标准品的研制提供基础。Objective To obtain standard strains of eight main serotypes of Salmonella and establish a process for screening standard strains according to the Technical Requirements for Evaluation of National Standard Strains of Pathogenic Microorganisms(WS/T 812—2022).Methods Morphological observation,biochemical testing,and genome sequencing analysis were conducted on 24 strains of initial and passaged Salmonella to obtain standard strains with typical morphology and biochemistry,and stable genomic characteristics.Results The results showed that 8 strains belonging to 8 serotypes(S.Typhi,S.Paratyphi A,S.Paratyphi B,S.Paratyphi C,S.Typhimurium,S.Enteritidis,monophasic S.Typhimurium,and S.Choleraesuis)were selected from 24 Salmonella strains.These 8 strains had typical morphology and biochemical reactions on the recommended selective culture medium for Salmonella isolation,with minimal genomic variation after passage,and stable serum agglutination results,which can be used as reference strains for Salmonella testing.Conclusion This study obtained 8 main serotype standard strains of Salmonella with corresponding pathogenic characteristics through screening,which can serve as reference strains for Salmonella detection,serotyping quality control,and genome evolution analysis,thus provide a foundation for subsequently development of nucleic acid standard materials.

关 键 词：沙门菌 血清型 基因组 标准菌株 

分 类 号：R211[医药卫生—中医学] R378[生物学—微生物学] Q939.99