滇杨幼苗对镉、锌胁迫的生理响应与耐受性研究  

Study on Physiological Response and Tolerance of Populus yunnanensis Seedlings to Cadmium and Zinc Stress

作　　者：王艳霞[1,2] 郑武扬 侯磊[1] 刘楚天 何承忠 陈柱学 魏文雅 Wang Yanxia;Zheng Wuyang;Hou Lei;Liu Chutian;He Chengzhong;Chen Zhuxue;Wei Wenya(College of Ecology and Environment,Southwest Forestry University,Kunming Yunnan 650233,China;Key Laboratory of Ecological Environment Evolution and Pollution Control in Mountainous and Rural Areas of Yunnan Province,Kunming Yunnan 650233,China;College of Life Science,Southwest Forestry University,Kunming Yunnan 650233,China)

机构地区：[1]西南林业大学生态与环境学院,云南昆明650233 [2]西南林业大学云南省山地农村生态环境演变与污染治理重点实验室,云南昆明650233 [3]西南林业大学生命科学学院,云南昆明650233

出　　处：《西南林业大学学报（自然科学）》2024年第1期31-39,共9页Journal of Southwest Forestry University：Natural Sciences

基　　金：国家自然科学基金项目(31860219)资助;云南省山地农村生态环境演变与污染治理重点实验室开放基金项目(2020YB002)资助;云南省教育厅科学研究基金项目(2021Y232)资助。

摘　　要：为探究滇杨幼苗在Cd、Zn单一及复合胁迫下的生长、生理响应与耐受特征,采用土培盆栽的方式开展研究。结果表明:Cd、Zn单一及复合胁迫未显著改变滇杨株高、地径和总生物量,但单一Zn胁迫显著提高总根长、根表面积和根体积,Cd-Zn胁迫显著降低总根长;Zn可改善滇杨的苗木生长质量,Cd可导致滇杨生长变差,而Cd-Zn复合胁迫时生长状况最差。Cd、Zn单一胁迫未显著改变滇杨叶片的光合色素含量和净光合速率,Cd-Zn复合胁迫时叶绿素含量显著降低,但净光合速率和光响应特征参数变化不显著,而Cd单一胁迫时滇杨的R_(d)和LCP较CK显著增高,Zn单一胁迫时则LSP显著增高。Cd和Cd-Zn胁迫可激发滇杨氧化应激防御系统,使SOD和CAT活性显著提高,MDA和TPh含量无显著变化,Zn胁迫时SOD、CAT、MDA和TPh含量均无显著变化。滇杨幼苗对Cd、Zn单一及复合胁迫表现出耐受性,可作为Cd、Zn污染土壤生态修复的重要候选树种,其综合耐受值为Zn>CK>Cd>Cd-Zn。To investigate the growth and physiological responses of Populus yunnanensis,and to clarify its tolerance characteristics under cadmium(Cd),zinc(Zn) and their combined(Cd-Zn) stress,the experiment was carried out by soil culture pot.The results showed as follows:Cd and Zn stress did not significantly change plant height,ground diameter,and total biomass,but Zn stress significantly increased full root length,root surface area and root volume,while Cd-Zn stress significantly decreased full root length.Zn stress improved the nursery stock quality of P.yunnanensis,Cd stress made the growth worse,and Cd-Zn stress was the worst growth status.Photosynthetic pigment content and net photosynthetic rate were not changed by single stress of Cd and Zn.Under CdZn stress,chlorophyll content decreased significantly,while net photosynthetic rate and light response characteristic parameters did not change significantly.Dark respiration rate(R_d) and light compensation point(LCP) were significantly increased under Cd stress,and light saturation point(LSP) was significantly increased under Zn stress.Cd and Cd-Zn stress could stimulate the oxidative stress defense system of P.yunnanensis,the activities of superoxide dismutase(SOD) and catalase(CAT) were significantly increased,while the contents of malondialdehyde(MDA) and total phenol(TPh) were not significantly changed,and SOD,CAT,MDA and TPh were not significantly changed under Zn stress.The seedlings of P.yunnanensis showed tolerance to single and combined stress of Cd and Zn,and could be used as an important candidate tree for ecological remediation of Cd and Zn polluted soil.The total tolerance values(D) of all treatments followed the sequence of Zn>CK>Cd> Cd-Zn.

关 键 词：滇杨 镉、锌胁迫 生长 光合生理 重金属 

分 类 号：Q945.78[生物学—植物学]