水稻DEP1突变体籽粒品质性状及其代谢组分析  

Grain Quality Characters and Metabolome Analysis of DEP1 Mutant in Rice

作　　者：赵明珠 王丽丽 马作斌 唐志强 郑文静 ZHAO Mingzhu;WANG Lili;MA Zuobin;TANG Zhiqiang;ZHENG Wenjing(Liaoning Rice Research Institute,Shenyang 110161,China;Crop Research Institute,Liaoning Academy of Agricultural Sciences,Shenyang 110161,China)

机构地区：[1]辽宁省水稻研究所,辽宁沈阳110161 [2]辽宁省农业科学院作物研究所,辽宁沈阳110161

出　　处：《华北农学报》2023年第S01期1-7,共7页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(31901526);辽宁省博士启动基金(2020-BS-299);现代农业产业技术体系建设专项(CARS-01-55);现代农业产业技术体系建设专项(CARS-01-50);辽宁省应用基础研究计划项目(2022JH2/101300283)。

摘　　要：为分析DEP1等位变异对水稻籽粒品质和籽粒代谢轮廓的影响。以优质粳稻品种稻花香及其直立穗型突变体dep1-1为试材,测定了籽粒的品质性状及差异代谢产物。结果表明,突变体dep1-1在DEP1基因第5外显子处存在1个单碱基A插入,导致翻译提前终止,从而使G蛋白γ亚基富含半胱氨酸的区域缺失。与野生型品种相比,突变体dep1-1的粒长、外观、黏度、平衡度、食味值、峰值、回复值和峰值时间等指标显著降低,而直链淀粉含量、酸度值和硬度显著提高。与野生型相比,突变体dep1-1中37个代谢物含量显著上调,而45个代谢物含量显著下调。KEGG分析表明,差异代谢物主要富集于半乳糖代谢、脂肪酸生物合成、淀粉蔗糖代谢、亚油酸代谢、ABC转运蛋白、类苯基丙烷生物合成等途径。其中,与野生型品种相比,突变体dep1-1籽粒的蔗糖、D-棉子糖、棉子糖、D-(+)-麦芽糖D-(+)、甜菜碱、吲哚含量显著提高,而硬脂酸、软脂酸、L-亮氨酸、吲哚乙酸含量显著下降。上述的差异代谢物初步解释了突变体dep1-1籽粒品质变化的生理生化机制。In order to determine the effect of allele variation in DEP1 locus on quality traits and metabolic profile of grains in rice,the high quality japonica cultivar Daohuaxiang and its erect panicle type mutant dep1-1 were used.The grain quality traits and differential metabolites between wild-type and mutant dep1-1 were measured.The results showed that the mutant dep1-1 had a single base A inserted into the fifth exon of the DEP1 gene,leading to premature termination of translation and the deletion of the cysteine-rich region in G-protein gamma subunit.Compared with wild-type cultivar,the grain length,appearance,viscosity,balance,taste value,peak value,recovery value and peak time of mutant dep1-1 were significantly decreased,while amylose content,acidity value and hardness were significantly increased.Compared with wild-type cultivar,37 metabolites were significantly up-regulated in dep1-1,while 45 were significantly down-regulated.KEGG analysis showed that these differential metabolites were mainly enriched in galactose metabolism,fatty acid biosynthesis,starch sucrose metabolism,linoleic acid metabolism,ABC transporters,and phenylpropanoid biosynthesis pathways.Compared with wild-type cultivar,the contents of sucrose,D-raffinose,raffinose,D-(+)-maltose D-(+),betaine and indole of grains significantly increased in dep1-1,while those of stearic acid,palmitic acid,L-leucine and indole-3-acetic acid significantly decreased.These differential metabolites preliminarily explained the physiological and biochemical mechanism of grain quality changes in dep1-1 mutant.

关 键 词：水稻 直立穗型 DEP1 代谢组 籽粒品质 

分 类 号：S511.03[农业科学—作物学]