四川凉山州3个黑绵羊群体Prdm9基因锌指序列的比较研究  

Comparative Study of the Zinc Finger Sequences of Prdm9 Gene in Three Black Sheep Populations in Liangshan Prefecture,Sichuan

作　　者：张芬芬 黄卫平 黄林[1] 金素钰[1] 郑玉才[1] ZHANG Fenfen;HUANG Weiping;HUANG Lin;JIN Suyu;ZHENG Yucai(College of Animal&Veternary Sciences,Southwest Minzu University,Chengdu 610041,China;Sichuan Liangshan Yi Autonomous Prefecture Institute of Animal Science,Xichang 615042,China)

机构地区：[1]西南民族大学畜牧兽医学院,四川成都610041 [2]四川省凉山彝族自治州畜牧科学研究所,四川西昌615042

出　　处：《华北农学报》2023年第S01期408-412,共5页Acta Agriculturae Boreali-Sinica

基　　金：四川省凉山州科技计划项目(18NYCX0029)。

摘　　要：旨在通过测定Prdm9基因锌指域序列,探索四川3个黑绵羊群体间的亲缘关系。采集黑绵羊群体抗凝全血,提取基因组DNA,用PCR特异扩增Prdm9基因锌指域,进一步通过克隆测序以分析遗传多样性。结果显示,黑绵羊中鉴定出存在9种锌指,锌指间存在1~5个氨基酸差异,共构成5种Prdm9锌指域,分别定义为不同的等位基因(A、B、C、D和E),其中A~D包含8个锌指,这是试验黑绵羊Prdm9锌指域的主要类型,E包含9个锌指且仅发现于盐源黑绵羊中,其等位基因频率仅为0.050。这些等位基因形成6种基因型,包括AA、AC、BB、BD、CC和EE,在布拖黑绵羊和普格黑绵羊中均检测到4种基因型,而盐源黑绵羊中有6种,且BB和EE仅在该群体中观察到。Prdm9的等位基因频率和PIC值在3个黑绵羊群体中存在不同程度差异,有较丰富的遗传多态性,其中盐源黑绵羊相对最丰富。3个黑绵羊群体Prdm9锌指域的等位基因频率分布均极显著偏离哈代-温伯格平衡(P<0.01),提示它们在育种中受到较大的人工选择影响。综上所述,黑绵羊群体中Prdm9有较丰富的遗传多样性,3个群体间亲缘关系均较近。It aimed to explore the kinship between three black sheep populations in Sichuan by determining the sequence of the zinc finger domain of Prdm9.Anticoagulated whole blood was collected from the black sheep population,genomic DNA was extracted,and the zinc finger domain of the Prdm9 was specifically amplified by PCR,and further sequenced by cloning to analyze genetic diversity.The results showed that nine types of zinc fingers were identified in black sheep,with 1-5 amino acid differences between zinc fingers,constituting five Prdm9 zinc finger domains,defined as different alleles(A,B,C,D and E),of which A to D contained eight zinc fingers,which were the main types of Prdm9 zinc finger domains in the tested black sheep,and E contained nine zinc fingers and was only found in Yanyuan black sheep,with an allele frequency of 0.050.These alleles formed six genotypes,including AA,AC,BB,BD,CC and EE,and four genotypes were detected in both Butuo and Puge black sheep,while six were found in Yanyuan black sheep,and BB and EE were only observed in this population.The Prdm9 allele frequencies and PIC values varied among the three black sheep populations,with richer genetic polymorphisms,of which the Yanyuan black sheep were relatively the most abundant.The allele frequency distribution of the Prdm9 zinc finger domain in all three black sheep populations deviated significantly from Hardy-Weinberg equilibrium(P<0.01),suggesting that they were influenced by artificial selection in breeding.In conclusion,Prdm9 has rich genetic diversity in the black sheep population,and all three populations are closely related to each other.

关 键 词：黑绵羊 Prdm9 锌指 克隆测序 亲缘关系 

分 类 号：S826[农业科学—畜牧学]