新疆裸重唇鱼3种同工酶的电泳分析  

Electrophoretic Analysis on 3 Kinds of Isozymes in Gymnodiptychus dybowskii

作　　者：张涛[1] 陈鹏 沙文军 王冉旋 何力[1] 牛建功[2] 甘金华[1] ZHANG Tao;CHEN Peng;SHA Wenjun;WANG Ranxuan;HE Li;NIU Jiangong;GAN Jinhua(Laboratory of Quality&Safety Risk Assessment for Aquatic Products(Wuhan),Ministry of Agriculture and Rural Affairs,Freshwater Fish Germplasm Quality Supervision and Testing Center,Ministry of Agriculture and Rural Affairs,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Wuhan 430223,China;Xinjiang Uygur Autonomous Fishery Research Institute,Urumqi 830000,China;National Energy Group Xinjiang Jilintai Hydropower Development Co.,Ltd,Yining 835100,China)

机构地区：[1]农业农村部水产品质量安全风险评估实验室(武汉),农业农村部淡水鱼类种质监督检验测试中心,中国水产科学研究院长江水产研究所,湖北武汉430223 [2]新疆维吾尔自治区水产科学研究所,新疆乌鲁木齐830000 [3]国家能源集团新疆吉林台水电开发有限公司,新疆伊宁835100

出　　处：《华北农学报》2023年第S01期444-451,共8页Acta Agriculturae Boreali-Sinica

基　　金：中央级公益性科研院所基本科研业务费专项资金资助(YFI202202);国家产地水产品兽药残留监控计划(2022);内江师范学院长江上游鱼类资源保护与利用四川省重点实验室开放课题(NJTCCJSYSYS11)。

摘　　要：为了解新疆裸重唇鱼的生化遗传特性,丰富新疆裸重唇鱼种质资源研究的内容,借助聚丙烯胺凝胶对新疆裸重唇鱼5种组织(心脏、眼睛晶状体、肝脏、肾脏和脾脏)中的乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)以及酯酶(EST)进行了电泳分析。结果表明:新疆裸重唇鱼的LDH、MDH和EST同工酶系统具有个体差异性和组织特异性。组织特异性主要表现在同工酶位点表达及其表达活性程度不同两方面,可能受遗传和代谢双重调控,与各组织执行生理功能密切相关。LDH-C基因只在肝脏中表达,酶谱图上靠近阴极附近,为典型“肝带”。新疆裸重唇鱼同工酶个体差异性可能是其在漫长的进化过程中为了能适应特殊生境的需要。新疆裸重唇鱼心脏中上清液型苹果酸脱氢酶(s-MDH)最多表达出6条酶带,可能是新疆裸重唇鱼在进化过程中四倍体化使基因重复的结果。研究结果可从生化遗传角度为新疆裸重唇鱼种质资源保护利用提供一定理论依据。This paper was to learn about the biochemical heredity characteristics and to enrich the research data about the germplasm resources of Gymnodiptychus dybowskii.Lactate dehydrogenase(LDH)isozymes,malate dehydrogenase(MDH)isozymes and Esterase(EST)isozymes in five kinds of tissues(heart,crystalline lens,liver,kidney and spleen)of Gymnodiptychus dybowskii were preliminarily studied by using the vertical polyacrylamide gel electrophoresis.The results showed that:LDH isozymes,MDH isozymes and EST isozymes showed tissue specificity and individual variations.The tissue specificity mainly reflected in expression of isozyme sites and degree of isozyme activity,which may be regulated by both heredity and metabolism and were related to the specific physiological functions of various kinds of tissues.LDH-C gene only expressed in liver.Its isozyme bands were near to negative pole in electrophoretogram,which were typical liver bands.In order to adapt the unique habitant,the isozymes of Gymnodiptychus dybowskii showed individual variations.Six supernate type of MDH isozyme bands expressed in heart may be the result of gene redundancy due to tetraploid during the evolution of Gymnodiptychus dybowskii.The research results could provide theoretical basis for the conservation and utilization of germolasm resources for Gymnodiptychus dybowskii from the biochemical genetics level.

关 键 词：新疆裸重唇鱼 乳酸脱氢酶 苹果酸脱氢酶 酯酶 组织特异性 个体差异性 

分 类 号：S917.4[农业科学—水产科学]