富含萝卜硫素的西兰花提取物对小鼠外周血CD8^(+)T淋巴细胞活化及肠道菌群的影响  

Effect of sulforaphane-rich broccoli extract on intestinal microbiota and the activation of peripheral blood CD8+T lymphocytes of the mouse

作　　者：王政 申春一 黄建敏[1] 刘金燕[1] 李洁瑶[2] 张毅[1,2] WANG Zheng;SHEN Chunyi;HUANG Jianmin;LIU Jinyan;LI Jieyao;ZHANG Yi(Biotherapy Center,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052;Department of Oncology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052)

机构地区：[1]郑州大学第一附属医院生物细胞治疗中心,郑州450052 [2]郑州大学第一附属医院肿瘤科,郑州450052

出　　处：《郑州大学学报（医学版）》2024年第1期35-39,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金项目(81702812);河南省中央引导地方科技发展资金项目(Z20221343036)。

摘　　要：目的:探讨富含萝卜硫素(SFN)的西兰花提取物对小鼠肠道菌群及外周血CD8^(+)T淋巴细胞活化的影响。方法:将15只C57BL/6N小鼠随机分为空白对照(CON)组、阴性对照(NC)组和SFN组,每组5只小鼠,分别给予蒸馏水、不含SFN的西兰花提取物、含0.3 g/L SFN的西兰花提取物125μL灌胃,连续4周。每2 d测量1次体重。实验结束前采集小鼠外周血检测活化的CD8^(+)T淋巴细胞百分比以及CD8^(+)T淋巴细胞中IL-2、TNF-α的含量。收集小鼠粪便,进行肠道菌群分析。结果:3组小鼠不同时间点体重差异无统计学意义(P>0.05)。SNF组小鼠外周血活化的CD8^(+)T淋巴细胞百分比、CD8^(+)T淋巴细胞中IL-2和TNF-α含量高于CON组和NC组(P<0.05)。3组小鼠肠道菌群组成存在差异,SFN组小鼠肠道中疣微菌门及阿克曼菌属的相对丰度高于CON组和NC组(P<0.05)。肠道中阿克曼菌属的相对丰度与CD8^(+)T淋巴细胞中IL-2和TNF-α含量呈正相关(r=0.934和0.664,P<0.05)。结论:长期连续服用富含SFN的西兰花提取物能增加小鼠肠道中阿克曼菌属等有益菌的丰度,同时可增强外周血CD8^(+)T淋巴细胞的功能。Aim:To explore the impact of broccoli extract with sulforaphane(SFN)on the mouse intestinal microbiota and the activation of peripheral blood CD8^(+)T lymphocytes.Methods:A total of 15 C57BL/6N mice were randomly allocated into blank control(CON)group,negative control(NC)group,and SFN group,with 5 mice in each group.The mice were administered distilled water,broccoli extract without SFN,or broccoli extract with 0.3 g/L SFN by gavage,respectively,for 4 weeks consecutively.Body weight was measured every 2 days.Peripheral blood was collected at the end of the experiment to assess the percentage of activated CD8^(+)T lymphocytes and the levels of IL-2 and TNF-αin the CD8^(+)T lymphocytes.Fecal samples were collected for analysis of the intestinal microbiota.Results:There was no statistical difference in body weight among the three groups(P>0.05).The percentage of activated CD8^(+)T lymphocytes and the levels of IL-2 and TNF-αin the CD8^(+)T lymphocytes were higher in the SFN group than those in the CON and NC groups(P<0.05).The composition of the intestinal microbiota differed among the groups,with higher relative abundances of Verrucomicrobia and Akkermansia in the SFN group compared with the CON and NC groups(P<0.05).The relative abundance of Akkermansia in the intestine was positively correlated with the levels of IL-2 and TNF-α(r=0.934 and 0.664,P<0.05).Conclusion:Long-term continuous consumption of broccoli extract rich in SFN can increase the relative abundance of beneficial bacteria such as Akkermansia,in the mouse intestine,and enhance the function of peripheral blood CD8^(+)T lymphocytes.

关 键 词：萝卜硫素 肠道菌群 CD8^(+)T淋巴细胞 小鼠 

分 类 号：R459.3[医药卫生—治疗学]