玉米须总黄酮抗大鼠高尿酸血症及肾脏保护作用  

Anti-hyperuricemia and renal protection effects of total flavonoids from corn silk

作　　者：黄宇涵 徐莎 房伟[3] 宋玮[2] 贺雅静[4] 张玉霞[5] 马振勇[5] HUANG Yuhan;XU Sha;FANG Wei;SONG Wei;HE Yajing;ZHANG Yuxia;MA Zhenyong(Department of Pharmacy,Shangqiu Medical College,Shangqiu,Henan 476005;Department of Basic Medical,Shangqiu Medical College,Shangqiu,Henan 476005;Department of Pharmacy,the First Affiliated Hospital,Henan University of Traditional Chinese Medicine,Zhengzhou 450000;Department of Pathology,the First People′s Hospital of Shangqiu,Shangqiu,Henan 476100;Department of Clinical Medicine,Shangqiu Medical College,Shangqiu,Henan 476005)

机构地区：[1]商丘医学高等专科学校药学院,河南商丘476005 [2]商丘医学高等专科学校基础医学院,河南商丘476005 [3]河南中医药大学第一附属医院药学部,郑州450000 [4]商丘市第一人民医院病理科,河南商丘476100 [5]商丘医学高等专科学校临床医学院,河南商丘476005

出　　处：《郑州大学学报（医学版）》2024年第1期45-50,共6页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省高等学校重点科研项目(21B310007)。

摘　　要：目的:探讨玉米须总黄酮(TFCS)抗大鼠高尿酸血症(HUA)及肾脏保护的价值。方法:将60只SD大鼠随机分成正常对照组,模型组,别嘌呤醇组和TFCS低、中、高剂量组,每组10只。除正常对照组外,其余各组给予250 mg/(kg·d)乙胺丁醇和100 mg/(kg·d)腺嘌呤的混悬液灌胃14 d,构建大鼠HUA模型。造模后别嘌呤醇组给予50 mg/(kg·d)别嘌呤醇,TFCS低、中、高剂量组分别给予0.5、1.0、2.0 mg/(kg·d)TFCS灌胃14 d。观察并记录大鼠的一般状态和体重;检测大鼠血清中尿酸(UA)、肌酐(Cr)、尿素氮(BUN)、黄嘌呤氧化酶(XOD)水平及尿液中UA水平;HE染色观察肾组织病理形态变化;免疫组化法检测大鼠肾组织尿酸转运体1(URAT1)蛋白的表达;qRT-PCR法检测大鼠肾组织葡萄糖转运体9(GLUT9)mRNA的表达。结果:与正常对照组比较,模型组大鼠精神状态萎靡、排尿增多;血清UA、Cr、BUN、XOD水平升高,尿液UA水平降低(P<0.05);肾小管上皮边界模糊,细胞肿胀变性,肾间质炎性细胞浸润;肾组织URAT1蛋白和GLUT9 mRNA表达水平升高(P<0.05)。与模型组比较,别嘌呤醇组和TFCS低、中、高剂量组大鼠精神状态及排尿情况好转;血清UA、Cr、BUN、XOD水平降低,尿液UA水平升高(P<0.05);肾组织损伤减轻,URAT1蛋白和GLUT9 mRNA表达水平降低(P<0.05)。结论:TFCS可有效降低HUA大鼠UA水平,并对肾脏有一定保护作用,其作用机制可能与抑制UA合成关键酶活性及调节UA转运蛋白表达有关。Aim:To explore the value of total flavonoids from corn silk(TFCS)in the treatment of hyperuricemia(HUA)and the protection of kidneys in rats.Methods:A total of sixty SD rats were randomly allocated into normal control group,model group,allopurinol group,and TFCS low,medium,and high-dose groups,with 10 rats in each group.The normal control group was given physiological saline by gavage,while the other groups were treated with 250 mg/(kg·d)ethambutol and 100 mg/(kg·d)adenine suspension daily to construct the rat HUA model.After molding,the allopurinol group was given 50 mg/(kg·d)allopurinol,while the low,medium,and high-dose TFCS groups were given 0.5,1.0,and 2.0 mg/(kg·d)TFCS by gavage for 14 days.The general state of rats was observed and recorded,and the weight of rats in each group was recorded.After the last administration,serum uric acid(SUA),creatinine(Cr),urea nitrogen(BUN),xanthine oxidase(XOD)levels,and urine uric acid(UUA)level were detected.HE staining was used to observe pathological changes in renal tissue.Immunohistochemical method was used to detect changes in uric acid transporter 1(URAT1)in rat kidney tissue.qRT-PCR was used to detect the expression level of the glucose transporter 9(GLUT9)gene in rat kidney tissue.Results:Compared with the normal control group,the model group rats showed a depressed mental state and increased urination,with higher SUA,Cr,BUN,and XOD,but lower UUA(P<0.05).The epithelial boundaries of renal tubules were blurred in the model group,with cell swelling and degeneration,and inflammatory cell infiltration in the renal interstitium.The expressions of URAT1 protein and GLUT9 mRNA increased in the model group(P<0.05).Compared with the model group,the rats in the allopurinol group and TFCS low,medium,and high-dose groups showed better mental state and urination condition,with lower SUA,Cr,BUN,XOD,but higher UUA(P<0.05).The renal tissue damage was alleviated,and the expressions of URAT1 protein and GLUT9 mRNA were reduced in these groups(P<0.05).Conclusion:TFCS can effect

关 键 词：玉米须总黄酮 高尿酸血症 大鼠 URAT1 GLUT9 

分 类 号：R285.5[医药卫生—中药学]