外泌体作为潜在肿瘤标志物的定量检测方法研究进展  

Research Progress in Quantitative Detection Methods of Exosomes as Potential Tumor Markers

作　　者：梁硕 邱宇飞 王译羽 徐晓筱 杜熙钦 王琛 邹秉杰 宋沁馨[1] LIANG Shuo;QIU Yufei;WANG Yiyu;XU Xiaoxiao;DU Xiqin;WANG Chen;ZOU Bingjie;SONG Qinxin(Key Laboratory of Drug Quality Control and Pharmacovigilance of Ministry of Education,China Pharmaceutical University,Nanjing 210009,China;The First School of Clinical Medicine,Southern Medical University,Nanjing 210002,China)

机构地区：[1]中国药科大学药物质量与安全预警教育部重点实验室,江苏南京210009 [2]南方医科大学第一临床医学院,江苏南京210002

出　　处：《药学进展》2023年第11期837-848,共12页Progress in Pharmaceutical Sciences

基　　金：国家自然科学基金项目(No.82173780);江苏省重点研发计划社会发展面上项目(No.BE2023843);药物质量与安全预警教育部重点实验室资助项目(No.DQCP20/21MS04);大学生创新创业训练计划项目(No.2023103161187)。

摘　　要：外泌体是一种直径大小为40~150 nm的细胞外囊泡,分布于几乎所有体液中。作为肿瘤液体活检的新靶标,癌细胞来源的外泌体包含多种肿瘤生物信息,对肿瘤早期诊断和预后评价有重要的应用价值。对外泌体蛋白的定量检测方法包括荧光法、比色法、电化学法、下一代测序、表面增强拉曼光谱法、表面等离子体共振法的研究进展进行综述。Exosomes are extracellular vesicles with the diameter of 40-150 nm,which are distributed in almost all body fluids.As a new target for tumor liquid biopsy,cancer cell-derived exosomes contain a variety of tumor biological information,making them valuable for early tumor diagnosis and prognosis evaluation.This paper reviews the research progress in quantitative detection methods of exosomal proteins.The methods discussed include fluorescence,colorimetry,electrochemistry,next-generation sequencing,surface-enhanced Raman spectroscopy,and surface plasmon resonance.

关 键 词：外泌体 定量检测 肿瘤标志物 液体活检 

分 类 号：R917[医药卫生—药物分析学]