检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:张琪[1] 李胜[1] 靳娟[1] 何建[1] 杨晓艳[1] 辛有全[1] 柏吉祥[1] 周奎章 张晓璐 蒋可 代瑞霞[1] Zhang Qi;Li Sheng;Jin Juan;He Jian;Yang Xiao-yan;Xin You-quan;Bai Ji-xiang;Zhou Kui-zhang;Zhang Xiao-u;Jiang Ke;Dai Rui-xia(Specialized Laboratory of Yersinia pestis,Qinghai Institute for Endemic Disease Prevention and Control,Xi'ning 810021;Department of Plague,Qinghai Institute for Endemic Disease Prevention and Control,Xi'ning 810021;Department of Public Health,Medical School,Qinghai University,Xi'ning 810001)
机构地区:[1]青海省地方病预防控制所鼠疫菌专业实验室,西宁810021 [2]青海省地方病预防控制所鼠疫预防控制科,西宁810021 [3]青海大学医学院公共卫生系,西宁810001
出 处:《中国抗生素杂志》2023年第10期1198-1200,I0001,共4页Chinese Journal of Antibiotics
基 金:国家自然科学基金项目(No.82260401)。
摘 要:目的 应用TaqMan-MGB探针实时荧光定量PCR技术快速检测青海省海西州鼠疫自然疫源地鼠疫菌耐链霉素基因,为今后该地区突发人间鼠疫的精准临床用药提供理论依据。方法 分离培养海西地区1957—2009年间取自鼠疫患者、媒介昆虫及中间宿主的代表性鼠疫菌110株,提取其DNA,针对我国链霉素耐药基因rpsl基因设计引物P-F和P-R和TaqMan-MGB探针Probe1 [FAM]和Probe2[VIC],利用荧光定量PCR技术,进行耐药rpsl基因筛查。结果 110株被试菌株中FAM检测均为阳性(RFU峰值>2000);VIC阳性的为0株(RFU峰值<200)。阳性对照和空白对照成立。结论 实时荧光定量PCR结果显示,该地区未检测出耐链霉素菌株。Objective To rapidly detect streptomycin resistance genes of Yersinia pestis in the Natural Focus of Plague of Haixi prefecture in Qinghai province by TaqMan MGB probe fluorescence real-time quantitative PCR,and to provide the theoretical basis for precise clinical drug use of plague emergencies in this area.Methods 110 representative strains of Yersinia pestis collected from 1957 to 2009 from plague patients,vector insects and intermediate hosts were isolated and cultured,with their DNA being extracted.Probe1[FAM]and Probe2[VIC],which were probes of Primers P-F and P-R and TaqMan-MGB,were designed for the rpsl gene,which was a streptomycin resistance gene often found in China,and fluorescence quantitative PCR was utilized to screen the rpsl gene of plague resistance genes.Results In FAM detection,all 110 strains had positive value(RFU peak>2000).No strains were positive in VIC detection(RFU peak<200).The positive control and the blank control proved to be effective.Conclusion Results of real-time quantitative PCR demonstrated that streptomycin resistant strains were not detected in the tested strains in this area.
关 键 词:鼠疫菌 TAQMAN-MGB探针 荧光定量PCR技术 耐链霉素 青海省海西州
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:3.22.120.195