荧光RNA及其生物传感技术研究进展  

Progress on fluorescent RNA and fluorescent RNA-based biosensing technology

作　　者：左方婷 张雅强 杨慧敏[1,2] 杨弋 陈显军[1,2] Fangting Zuo;Yaqiang Zhang;Huimin Yang;Yi Yang;Xianjun Chen(Interdisciplinary Research Center of Optogenetics and Synthetic Biology,State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China;Shanghai Advanced Research Base of Cell Metabolism Genetics,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China;School of Bioengineering,East China University of Science and Technology,Shanghai 200237,China)

机构地区：[1]华东理工大学光遗传学与合成生物学交叉学科研究中心,生物反应器工程国家重点实验室,上海200237 [2]华东理工大学药学院,上海市细胞代谢光遗传学技术前沿科学研究基地,上海200237 [3]华东理工大学生物工程学院,上海200237

出　　处：《遗传》2024年第2期92-108,共17页Hereditas（Beijing)

基　　金：国家自然科学基金项目(编号:32121005,32150028,21937004,91857202,32250009);国家重点研发计划(编号:2022YFC3400100)资助。

摘　　要：荧光RNA技术是一种新兴的RNA标记技术,可用于活细胞RNA的原位实时标记与成像,对于人们理解RNA的功能和调控机制发挥着至关重要的作用。基于荧光RNA的生物传感技术可用于活细胞内小分子代谢物以及蛋白质等靶标的实时动态检测,为生命科学基础研究以及生物医学传感技术开发提供极具价值的工具。本文对遗传编码的荧光RNA的发展历程、荧光RNA技术在活细胞RNA成像,以及基于荧光RNA的生物传感技术在活细胞代谢物检测等方面的应用进行了介绍和总结,并对该领域的发展现状和未来发展方向展开讨论和展望,以期为该技术的进一步发展和在相关领域的应用提供参考。Fluorescent RNA is a kind of emerging RNA labeling technique that can be used for in situ labeling and imaging of RNA in live cells,which plays an important role in understanding the function and regulation mechanism of RNA.Biosensing technology based on fluorescent RNA can be applied in dynamic detection of small molecule metabolites and proteins in real time,offering valuable tools for basic life science research and biomedical sensing technology development.In this review,we introduce the development of genetically encoded fluorescent RNA,and the application of fluorescent RNA in RNA imaging and biosensing technology based on fluorescent RNA in biosensing in live cell.Meanwhile,we discuss the direction and challenge of future development of fluorescent RNA technology to provide valuable insights for further development and application of this technology in relevant fields.

关 键 词：荧光RNA 荧光标记 RNA成像 生物传感 

分 类 号：Q503[生物学—生物化学]