不同香菇菌株生长特性及优良菌株筛选  

Growth characteristics of different Lentinula edodes strains and screening of elite strains

作　　者：鲍红春 吴小燕 李小雷[2] 于传宗 庞杰 李文彪 王静 季祥 郭芳颖 孔令江 BAO Hongchun;WU Xiaoyan;LI Xiaolei;YU Chuanzong;PANG Jie;LI Wenbiao;WANG Jing;JI Xiang;GUO Fangying;KONG Lingjiang(Inner Mongolia Agricultural and Animal Husbandry Technology Promotion Center,Hohhot 010011,China;College of Agriculture,Inner Mongolia Agricultural University,Hohhot 010019,China;Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China)

机构地区：[1]内蒙古自治区农牧业技术推广中心,内蒙古呼和浩特010011 [2]内蒙古农业大学农学院,内蒙古呼和浩特010019 [3]内蒙古自治区农牧业科学院,内蒙古呼和浩特010031

出　　处：《北方农业学报》2023年第6期59-69,共11页Journal of Northern Agriculture

基　　金：内蒙古自治区科技计划项目(2020GG0223,2022YFHH0110)。

摘　　要：【目的】研究不同香菇菌株生长特性及菌株间差异性,筛选适宜内蒙古西部地区栽培的优良香菇菌株。【方法】以26个香菇菌株为试验材料,采用形态观察及拮抗试验的方法,对其菌落形态、菌丝生长速度、生长适宜温度、菌丝长势及菌株间亲缘关系进行分析。【结果】供试菌株最适生长温度为25℃,XG-168、XG-Y、冬香4号、冬香5号菌落边缘完整、菌丝生长速度快、菌落长势强、菌株锁状联合结构较多,菌丝平均生长速度均为3.33 mm/d,菌丝萌发时间均为2 d,菌丝长满时间均为12 d,菌丝长势均较强,菌丝均较浓密。XG-Y与阜平香菇0912、HXG-8L1与庆元香菇、遵化香菇168与平泉香菇168、XG-Y与MXG-1669、XG-12与MXG-1669菌株间亲缘关系较近,宝林2号与其他菌株亲缘关系远。【结论】XG-168、XG-Y、冬香4号、冬香5号菌株生长特性较为突出,可作为内蒙古西部地区优质菌株进行栽培。【Objective】Study the growth characteristics of different Lentinula edodes strains and the differences between strains to screen for the elite strains suitable for cultivation in the western regions of Inner Mongolia.【Methods】26 Lentinula edodes strains were used as test materials.The colony morphology,mycelium growth rate,optimum growth temperature,mycelial growth vigor,and phylogenetic relationships between strains were analyzed by morphological observation and antagonistic test.【Results】The optimum growth temperature of the test strains was 25℃.XG-168,XG-Y,Dongxiang No.4,and Dongxiang No.5 had complete colony edges,fast mycelial growth rate,vigorous colony growth,and multiple lock-like joint structure.The average mycelium growth rates were all 3.33 mm/d.Mycelium germination times were all 2 d.The full growth times of mycelium were all 12 d,with vigorous and dense mycelium growth.The genetic relationships between XG-Y and Fupingxianggu 0912,HXG-8L1 and Qingyuanxianggu,Zunhuaxianggu 168 and Pingquanxianggu 168,XG-Y and MXG-1669,XG-12 and MXG-1669 strains were relatively close.While Baolin No.2 had a distant genetic relationship with other strains.【Conclusion】XG-168,XG-Y,Dongxiang No.4 and Dongxiang No.5 had prominent growth characteristics,could be used as elite strains for cultivation in the western regions of Inner Mongolia.

关 键 词：香菇菌株 生长特性 拮抗反应 亲缘关系 

分 类 号：S646.12[农业科学—蔬菜学]