不同年龄段小鼠小脑浦肯野细胞胞体和树突形态的分析  

The analysis of somatic and dendritic morphology of cerebellar Purkinje cells in mice at different ages

作　　者：裴沛 汤正权 PEI Pei;TANG Zhengquan(School of Life Sciences,Anhui University,Hefei 230601,China)

机构地区：[1]安徽大学生命科学学院,合肥230601

出　　处：《生物学杂志》2024年第1期26-31,共6页Journal of Biology

基　　金：国家自然科学基金面上项目(82071061,32271059);安徽省留学回国人员创新创业项目(2020LCX036)。

摘　　要：为探讨小鼠小脑浦肯野细胞形态在不同年龄段的差异,挑选4个年龄段小鼠各5只,分别为14天龄、1月龄、2月龄和1年龄。选择小白蛋白作为浦肯野细胞独特的标记物,利用免疫组织化学方法,对该4组年龄段小鼠小脑的小白蛋白进行免疫荧光染色,在共聚焦显微镜下观察并比较浦肯野细胞胞体和树突形态。结果显示,2月龄小鼠浦肯野细胞树突长度显著高于其他年龄组(P<0.05),1年龄小鼠浦肯野细胞树突长度显著低于其他年龄组(P<0.05),浦肯野细胞胞体面积大小变化则呈现年龄段逐级递减趋势。研究同时分析小白蛋白在该4组年龄段小鼠小脑浦肯野细胞的荧光表达强度,其随年龄段呈现总体上升趋势。结果表明小脑浦肯野细胞胞体大小和树突长度与年龄相关,小白蛋白的表达与其发育呈相反趋势。研究结果提示,小白蛋白对小脑浦肯野细胞发育可能存在重要的调控作用。In order to investigate the difference in morphology of cerebellar Purkinje cells in mice at different ages,mice were divided into four age groups of five animals each corresponding to 14-day,1-month,2-month,and 1-year old mice.Parvalbumin selected as a unique marker for Purkinje cells,using immunochemistry,we observed and compared somatic and dendritic morphology of cerebellar Purkinje cells of these mice in four groups under a confocal microscope.The results showed that the dendritic length of cerebellar Purkinje cells in 2-month old mice was significantly higher than that in other groups(P<0.05),and the dendritic length of cerebellar Purkinje cells in 1-year old mice was significantly lower than that in other groups(P<0.05),as well as the somatic sizes of Purkinje cells of mice in 4 age groups showed a decreasing trend with age.In this study,we also analyzed the fluorescence intensity of parvalbumin in cerebellar Purkinje cells of mice in 4 age groups,which showed an overall increasing trend with age.Together,the results indicate that cell body size and dendritic length of cerebellar Purkinje are associated with age,with an opposite trend of parvalbumin expression and its development.This study suggests that parvalbumin may play an important role in regulation of cerebellar Purkinje cell development.

关 键 词：小脑 浦肯野细胞 小白蛋白 发育 免疫组化 

分 类 号：Q24[生物学—细胞生物学]