光眉刺蛾的线粒体基因组研究及系统发育分析  

The mitochondrial genome and phylogenetic analysis of Narosa fulgens(Leech,1889)

作　　者：林兴雨 宋南[1] LIN Xingyu;SONG Nan(College of Plant Protection,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]河南农业大学植物保护学院,郑州450002

出　　处：《生物学杂志》2024年第1期54-60,共7页Journal of Biology

基　　金：国家自然科学基金项目(U1904104)。

摘　　要：为探究光眉刺蛾Narosa fulgens(Leech,1889)的线粒体基因组结构及刺蛾科的系统发育关系,利用下一代测序方法获得光眉刺蛾线粒体全基因组序列。系统发育分析中使用斑蛾总科16个物种作为内群,并选择木蠹蛾科和网蛾科各1个物种作为外群,利用最大似然法和贝叶斯法重建刺蛾科的系统发育关系。光眉刺蛾的线粒体基因组包含37个基因(13个蛋白质编码基因、22个转运RNA基因和2个核糖体RNA基因)和1个非编码控制区,全长为15296 bp(GenBank登录号:OP919326)。光眉刺蛾线粒体基因组的13个蛋白质编码基因的起始密码子除cox1利用CGA开头外,其余蛋白质编码基因都是以ATN开头,终止密码子除cox2和nad4以不完整的T结尾外,其余蛋白质编码基因都是以TAA和TAG结尾。除trnS1因缺少DHU臂而形成一个简单的环,无法形成稳定的三叶草结构外,其余转运RNA基因均能形成典型的三叶草结构。rrnL的全长为1335 bp,A+T含量为85.24%。rrnS的全长为732 bp,A+T含量为84.70%。两个方法构建的系统发育树的结果均表明:斑蛾科和刺蛾科为单系群,光眉刺蛾与龟形小刺蛾Narosa nigrisigna为姐妹群。To explore the mitochondrial genome of Narosa fulgens(Leech,1889)and the phylogeny of Limacodidae,we newly sequenced the complete mitochondrial genome of N.fulgens(Lepidoptera,Limacodidae),by using the next-generation sequencing method.In phylogenetic analyses,we selected 16 exemplars of Zygaenoidea as ingroups.Each onespecies of Cossidae and Thyrididae was used as outgroup.We reconstructed the phylogenetic relationships using Maximum likelihood and Bayesian inference methods.The sequenced genome is a circular molecule of 15296 bp in length(GenBank accession No.OP919326)which possesses 13 protein-coding genes,22 transfer RNA genes,2 ribosomal RNA genes,and putative control regions as well.For most of the protein-coding genes,the star codons were ATN except for cox1 using CGA as star codon.Most of the protein-coding genes terminated with the stop codon TAA,while cox2 and nad4 employed the incomplete T as the stop codon.All tRNA genes can be folded into atypical cloverleaf structure,with the except of the trnS1-AGN.The trnS1 lacks the DHU arm.The lengths of the rrnL and rrnS genes were 1335 bp and 732 bp,respectively,with the A+T contents of 85.24%and 84.70%.Both phylogenetic inference methods showed largely identical tree topological structures:the families Limacodidae and Zygaenidae were respectively monophyletic,Narosa fulgens is sister to Narosa nigrisigna.

关 键 词：鳞翅目 刺蛾科 光眉刺蛾 线粒体基因组 系统发育 

分 类 号：Q754[生物学—分子生物学]