椴叶鼠尾草抗烟草花叶病毒活性成分研究  

Antiviral components of Salvia tiliifolia against tobacco mosaic virus

作　　者：王子夫 闫俊伊 刘洋[1] 吴江梅 闫晓慧[1] 胡世俊 WANG Zifu;YAN Junyi;LIU Yang;WU Jiangmei;YAN Xiaohui;HU Shijun(Key Laboratory of Forest Disaster Warning and Control in Yunnan Province,College of Biodiversity Conservation,Southwest Forestry University,Kunming 650224,China;College of Forestry,Southwest Forestry University,Kunming 650224,China)

机构地区：[1]西南林业大学生物多样性保护学院,云南省森林灾害预警与控制重点实验室,昆明650224 [2]西南林业大学林学院,昆明650224

出　　处：《生物学杂志》2024年第1期68-72,共5页Journal of Biology

基　　金：国家自然科学基金项目(32160378);云南省应用基础研究重点项目(2019FA011);云南省高层次人才项目(YNWR-QNBJ-2018-300)。

摘　　要：为寻找新型植物病毒抑制剂,以烟草花叶病毒(TMV)为供试病毒,研究发现入侵植物椴叶鼠尾草提取物具有抗TMV活性。通过半叶枯斑法不同施药方式筛选发现,在质量浓度为1 mg/mL时,石油醚萃取物的保护作用和钝化作用最强,抑制率为40.95%和52.85%;乙酸乙酯萃取物的治疗作用最强,抑制率为52.85%,与阳性对照药剂宁南霉素无显著差异。石油醚萃取物各馏分中,Fr7的保护作用和治疗作用最好,抑制率为40.51%和45.52%,Fr6的钝化作用最好,抑制率为74.63%。乙酸乙酯萃取物各馏分中,Fr1′的保护作用最强,抑制率为61.77%,Fr3′的治疗作用最强,抑制率为55.02%,Fr4′的钝化作用最强,抑制率为60.22%。运用现代色谱波谱技术分离鉴定了5个化合物分别为tilifodiolide、eupatoric acid、熊果酸、齐墩果酸和2-甲基-5,7-二羟基色原酮。In order to find new plant virus inhibitors,tobacco mosaic virus(TMV)was used as the test virus.It was found that the extract of Salvia tiliifolia,an invasive plant,showed anti-TMV activity.Screening by the half-leaf method along with different treatment pattern of extract,the petroleum ether extract showed the strongest protective effect and inactivation effect at the concentration of 1 mg/mL,with inhibition rate of 40.95%and 52.85%respectively.The ethyl acetate extract showed the best curative effect with the inhibition rate of 52.85%,which was not significantly different from Ningnanmycin,a positive control.Among fractions of petroleum ether extracts,Fr7 showed the best protective effect and curative effect,with the inhibition rate of 40.51%and 45.52%respectively,and Fr6 showed potent inactive effect with the inhibition rate of 74.63%.Among fractions of ethyl acetate extracts,Fr1′showed the strongest protective effect with an inhibition rate of 61.77%,Fr3′displayed the best curative effect with an inhibition rate of 55.02%,and Fr4′showed potent inactive effect with the inhibition rate of 60.22%.Five compounds were separated and identified by modern chromatographic spectrum technologies,they were tilifodiolide,eupatoric acid,ursolic acid,oleanolic acid,and 2-methyl-5,7-dioxy chromone.

关 键 词：椴叶鼠尾草 化学成分 分离纯化 烟草花叶病毒(TMV) 抗TMV活性 

分 类 号：Q939.4[生物学—微生物学] S432[农业科学—植物病理学] S482.1[农业科学—农业昆虫与害虫防治]