荧光聚合酶链反应法用于新型冠状病毒核酸检测扩增产物污染治理的相关应用  

Related application on pollution treatment of amplification products in novel coronavirus nucleic acid detection by real-time polymerase chain reaction

作　　者：闫晓枫[1] 李庆伟[1] 孙宇强 荣维娜[1] Yan Xiaofeng;Li Qingwei;Sun Yuqiang;Rong Weina(Department of Experimental Diagnosis,Heilongjiang Provincial Hospital,Haerbin 150036,Heilongjiang,China)

机构地区：[1]黑龙江省医院实验诊断部,黑龙江哈尔滨150036

出　　处：《实用检验医师杂志》2023年第4期372-376,共5页Chinese Journal of Clinical Pathologist

基　　金：黑龙江省卫生健康委科研课题(20211111000166)。

摘　　要：目的探讨荧光聚合酶链反应(PCR)法用于新型冠状病毒(新冠病毒)核酸检测时扩增产物污染治理的相关应用。方法建立新冠病毒基因检测扩增产物污染模型、物面污染模型以及空间(气溶胶)污染模型,比较不同化学试剂及物理紫外线照射治理方式对物面污染和空间污染的清除效果。结果75%乙醇消毒剂组、核酸清除剂组和不同浓度有效氯制剂组的循环阈值(CT值)均明显高于对照组,其中核酸清除剂组的CT值最高,玻璃、塑料、金属材质的CT值分别为34.74±2.63、34.68±3.25、35.71±3.07。500、1000、1500、2000、2500 mg/L有效氯制剂组对不同材质的CT值均高于75%乙醇消毒剂组,且有效氯的浓度越高,CT值越大,差异均有统计学意义。不同时长紫外线照射各组的CT值均明显高于对照组;其中以紫外线照射120 min组的CT值最高,玻璃、塑料、金属材质的CT值分别为35.51±2.70、35.49±2.63、35.55±2.58,且紫外线照射时长越长,CT值越大,差异均有统计学意义。治理后的通风+紫外线照射120 min+75%乙醇消毒剂+核酸清除剂+2500 mg/L有效氯制剂组的CT值为36.98±3.47,明显高于治理前及其他各组,差异均有统计学意义。结论荧光PCR法用于新冠核酸检测时扩增产物污染的治理方式较多,对物面污染的治理方式可选择核酸清除剂及2500 mg/L有效氯制剂,以及紫外线照射120 min,对空间污染环境的治理方式可选择通风+紫外线照射120 min+75%乙醇消毒剂+核酸清除剂+2500 mg/L有效氯制剂联合应用,值得重视。Objective To explore the related application of fluorescent polymerase chain reaction(PCR)in the treatment of amplification product pollution in detection of novel coronavirus(SARS-CoV-2)nucleic acid.Methods The models of SARS-CoV-2 gene detection amplification products pollution,physical surface pollution and space(aerosol)pollution were established to compare the removal effects of different chemical reagents and physical ultraviolet(UV)radiation treatments on physical surface pollution and space pollution.Results The circulation threshold(CT)value of 75%ethanol disinfectant group,nucleic acid scavenger group and different concentration of effective chlorine preparation groups were higher than that in control group,and the CT values of nucleic acid scavenger group were the highest,with CT values for glass,plastic and metal materials of 34.74±2.63,34.68±3.25 and 35.71±3.07,respectively.The CT values of different materials in 500,1000,1500,2000 and 2500 mg/L effective chlorine formulation groups were higher than those in 75%ethanol disinfectant group,and the higher the concentration of effective chlorine,the greater the CT values,with statistically significant differences.The CT values of each group exposed to long-term UV radiation at different time were higher than those of control group,and among them,the CT values of 120 minute UV irradiation group were the highest,with the CT values for glass,plastic and metal materials of 35.51±2.70,35.49±2.63 and 35.55±2.58,respectively.The longer the UV irradiation time,the larger the CT values,with statistically significant differences.The CT value of the ventilation+UV irradiation for 120 minutes+75%ethanol disinfectant+nucleic acid scavenger+2500 mg/L effective chlorine preparation group after treatment was 36.98±3.47,which was significantly higher than that before treatment and other groups,and the differences were statistically significant.Conclusions There are many amplified products,but nucleic acid scavenger,2500 mg/L effective chlorine and 120 minutes coul

关 键 词：聚合酶链反应 新型冠状病毒 核酸检测 扩增产物 污染治理 

分 类 号：R511[医药卫生—内科学] R440[医药卫生—临床医学]