HPLC法同时测定补肾活血散结胶囊中12种成分的含量  

Simultaneous content determination of twelve constituents in Bushen Huoxue Sanjie Capsules by HPLC

作　　者：尹继瑶 胡静 沈霞[1] 崔小敏[2] 任慧[2] 曲彤 李宁 鲁文静[2] 陈志永 屈凯[1,3] YIN Ji-yao;HU Jing;SHEN Xia;CUI Xiao-min;REN Hui;QU Tong;LI Ning;LU Wen-jin;CHEN Zhi-yong;QU Kai(Shaanxi University of Chinese Medicine,Xianyang 712083,China;Shaanxi Provincial Academy of Traditional Chinese Medicine,Xi’an 710003,China;Shaanxi Provincial Hospital of Traditional Chinese Medicine,Xi’an 710003,China)

机构地区：[1]陕西中医药大学,陕西咸阳712083 [2]陕西省中医药研究院,陕西西安710003 [3]陕西省中医医院,陕西西安710003

出　　处：《中成药》2024年第1期1-6,共6页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(81603454);国家中医药管理局中医药科学技术研究专项课题(GZY-KJS-2021-005);全国中医临床特色技术传承骨干人才项目(国中医药人教函[2019]36号);陕西省中医药中青年科技骨干人才项目(11610000MB29656749/2020-00894);陕西中医药大学校级创新项目(2021CX17)。

摘　　要：目的建立HPLC法同时测定补肾活血散结胶囊中没食子酸、原儿茶酸、莫诺苷、马钱苷、獐牙菜苷、芍药苷、金丝桃苷、紫云英苷、丹酚酸B、丹酚酸A、朝藿定C、淫羊藿苷的含量。方法分析采用Agilent 5 TC-C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长240 nm。结果12种成分在各自范围内线性关系良好(r≥0.9998),平均加样回收率97.11%~101.14%,RSD 0.60%~2.65%。结论该方法简便准确,重复性好,可用于补肾活血散结胶囊的质量控制。AIM To establish an HPLC method for the simultaneous content determination of gallic acid,protocatechuic acid,morroniside,loganin,sweroside,paeoniflorin,hypericin,astragalin,salvianolic acid B,salvianolic acid A,epimedin C and icariin in Bushen Huoxue Sanjie Capsules.METHODS The analysis was performed on a 30℃thermostatic Agilent 5 TC-C_(18)column(250 mm×4.6 mm,5μm),with the mobile phase comprising of acetonitrile-0.1%phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 240 nm.RESULTS Twelve constituents showed good linear relationships within their own ranges(r≥0.9998),whose average recoveries were 97.11%-101.14%with the RSDs of 0.60%-2.65%.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Bushen Huoxue Sanjie Capsules.

关 键 词：补肾活血散结胶囊 化学成分 含量测定 HPLC 

分 类 号：R927[医药卫生—药学]