UPLC-MS/MS法同时测定透骨香中6种成分的含量  

Simultaneous content determination of six constituents in Gaultheria leucocarpa var.yunnanensis by UPLC-MS/MS

作　　者：金林 周祖英 周家华 迟明艳 郑林[1,3] 黄勇 黄闻[4] JIN Lin;ZHOU Zu-ying;ZHOU Jia-hua;CHI Ming-yan;ZHENG Lin;HUANG Yong;HUANG Wen(Guizhou Provincial Key Laboratory for Pharmaceutics/State Key Laboratory for Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang 550004,China;College of Pharmaceutical Sciences,Guizhou Medical University,Guiyang 550004,China;National Engineering Research Center for Miao’s Medicines,Guiyang 550004,China;Burn Plastic Surgery Department,Affiliated Hospital of Guizhou Medical University,Guiyang 550001,China)

机构地区：[1]贵州医科大学,贵州省药物制剂重点实验室/药用植物功效与利用国家重点实验室,贵州贵阳550004 [2]贵州医科大学药学院,贵州贵阳550004 [3]国家苗药工程技术研究中心,贵州贵阳550004 [4]贵州医科大学附属医院烧伤整形外科,贵州贵阳550001

出　　处：《中成药》2024年第1期22-28,共7页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金(81960763,U1812403);贵州省科技计划项目(黔科合中引地[2023]006号,黔科合平台人才-GCC[2022]031-1号);2021年贵州省大学生创新创业训练计划项目(S202110660047)。

摘　　要：目的建立UPLC-MS/MS法同时测定透骨香中原儿茶酸、表儿茶素、绿原酸、槲皮苷、白珠树苷、滇白珠苷A的含量。方法分析采用Waters BEH C_(18)色谱柱(2.1 mm×100 mm,1.7μm);流动相水(含0.1%甲酸)-乙腈(含0.1%甲酸),梯度洗脱;体积流量0.3 mL/min;柱温40℃;电喷雾离子源;正负离子扫描;多反应监测模式。采用层次聚类分析(HCA)、主成分分析(PCA)筛选影响药材质量的重要成分。结果6种成分在各自范围内线性关系良好(r≥0.9982),平均加样回收率98.76%~101.88%,RSD 1.0%~2.5%。透骨香根、地上部分所含成分有较大差异,白珠树苷、表儿茶素、原儿茶酸是质量标志成分。结论该方法准确、高效,可用于透骨香的质量控制。AIM To establish an UPLC-MS/MS method for simultaneous content determination of protocatechuic acid,epicatechin,chlorogenic acid,quercitrin,gaultherin and gaultheroside A in Gaultheria leucocarpa var.yunnanensis.METHODS The analysis was performed on a 40℃thermostatic Waters BEH C_(18) column(100 mm×2.1 mm,1.7μm),with the mobile phase comprising of water(containing 0.1%formic acid)-acetonitrile(containing 0.1%formic acid)flowing at 0.3 mL/min in a gradient elution manner,and electron spray inoization source was adopted in positive and negative ion scanning with multiple reaction monitoring(MRM)mode.Hierarchical cluster analysis(HCA)and principal component analysis(PCA)was used to screen important components that affect the quality of medicinal materials.RESULTS Six constituents showed good linear relationships within their own ranges(R 2≥0.9982),whose average recoveries were 98.76%-101.88%with the RSDs of 1.0%-2.5%.The constituents of G.leucocarpa in the roots and aerial parts were quite different.Gaultherin,epicatechin and protocatechuic acid may be the quality mark constituents of G.leucocarpa.CONCLUSION This accurate and efficient method can be used for the quality control of G.leucocarpa.

关 键 词：透骨香 化学成分 含量测定 UPLC-MS/MS 层次聚类分析(HCA) 主成分分析(PCA) 

分 类 号：R284[医药卫生—中药学]