全州禾花鲤鳞片下层组织的结构及全长转录组分析  

Tissue Structure and Full-Length Transcriptome Analysis of the Scaly Sublayer of Cyprinus carpio var.Quanzhounensis

作　　者：杜雪松 文露婷 许艺兰 覃俊奇 黄姻 林勇 周康奇 潘贤辉 邓潜 陈忠 DU Xuesong;WEN Luting;XU Yilan;QIN Junqi;HUANG Yin;LIN Yong;ZHOU Kangqi;PAN Xianhui;DENG Qian;CHEN Zhong(Guangxi Key Laboratory of Aquatic Genetic Breeding and Health Aquaculture,Guangxi Academy of Fishery Sciences,Nanning 530021,China;Guangxi Key Laboratory of Veterinary Biotechnology,Guangxi Veterinary Research Institute,Nanning 530001,China)

机构地区：[1]广西壮族自治区水产科学研究院、广西水产遗传育种与健康养殖重点实验室,广西南宁530021 [2]广西壮族自治区兽医研究所、广西兽医生物技术重点实验室,广西南宁530001

出　　处：《渔业科学进展》2024年第1期70-84,共15页Progress in Fishery Sciences

基　　金：广西自然科学基金(2020GXNSFBA297146);国家自然科学基金(31960730);广西特色淡水鱼产业创新团队(nycytxgxcxtd-2021-08)共同资助。

摘　　要：为研究全州禾花鲤(Cyprinus carpio var.Quanzhounensis)皮肤组织结构和转录组差异、丰富鲤鱼皮肤转录组信息,选用建鲤(Cyprinus carpio var.Jian)和全州禾花鲤鳞片下层组织,利用透射电镜观察结构差异,发现禾花鲤与建鲤相比组织缺乏层叠排列的鸟嘌呤结晶且黑色素颗粒增多;利用牛津纳米孔(ONT)测序技术分析了转录组特征,获得高质量数据18.49Gb,识别为全长序列17182183条,检测出可变剪接(AS)事件3075个、可变多聚腺苷酸化(APA)57624个,预测新基因编码区序列15615个、长链非编码RNA(lncRNA)771个。其中,可变剪接事件中外显子跳跃、内含子保留数量在种间差异极显著(P<0.01),具有5个多聚腺苷酸化位点的转录本数量在种间差异极显著(P<0.01),表明可变剪接和多聚腺苷酸化参与性状形成相关的调控过程;共筛选出差异表达转录本841个,KEGG通路分析表明其在细胞外基质–受体相互作用、粘着斑等通路富集,GO分析发现其在细胞外隙、转录因子复合体、整合素复合物等词条显著富集,且最显著富集的KEGG通路和GO词条均与细胞外基质紧密关联,推测这些转录调控的变化可能导致皮肤细胞外基质的组成、密度和构象变化。后续研究应更多关注鸟嘌呤结晶缺失造成的皮肤性状变化,有助于全州禾花鲤种质资源开发与利用。Cyprinus carpio var.Quanzhounensis,native to Quanzhou County,Guilin City,Guangxi,has a dark brown body color,translucent gill cover,and abdominal skin,and is an important farmed species in the local integrated rice-fishery industry.A comparative study on the skin of Cyprinus carpio var.Quanzhounensis revealed a lack of reflective guanine crystals on the body surface and a significantly higher melanin content than that of C.carpio var.Jian,which was tentatively considered the direct cause of variations in body color of this group.Iridocytes are a pigment cell species that contain regularly arranged guanine crystals,which are the key material basis for the metallic luster of the fish body surface.In species such as medaka(Oryzias latipes)and zebrafish(Danio rerio),the absence of guanine crystals is considered a manifestation of the absence of iridocyte differentiation and therefore is ideal for studying the mechanism of pigment cell differentiation.The pnp4a,Gbx2,sox10,tfec genes and other iridocyte-related genes have been mined using mutant materials,and we have uncovered the differentiation mechanism that regulates the formation of iridescent cells.C.carpio var.color has abundant genetic variation in body color and is a good material for studying the mechanism of body color determination in fish.The roles of ASIP and MC1R in the aggregation and distribution of melanin and formation of black spots in C.carpio var.color were verified.The guanine crystalline deletion trait of C.carpio var.Quanzhounensis may be loaded with regulatory mechanism diversity and mutation loci related to guanine crystal formation or iridescent cell differentiation.In addition,as a rice-fish culture species,the living environment of C.carpio var.Quanzhounensis harvestmen differ significantly from pond and net-pen culture species,requiring high resistance to disease,adversity,and transport.It is unclear whether the absence of guanine crystals in their skin leads to changes in their basal physiological state,and in-depth studies are beneficia

关 键 词：全州禾花鲤 皮肤 性状 全长转录组 

分 类 号：S917.4[农业科学—水产科学]