莱氏拟乌贼输卵管腺超显微结构  

Microstructure and Ultrastructure of the Oviducal Gland of Sepioteuthis lessoniana

作　　者：王峥 刘长琳[1,2] 金广海 翟介明 林铃生 陈四清 WANG Zheng;LIU Changlin;JIN Guanghai;ZHAI Jieming;LIN Lingsheng;CHEN Siqing(Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Marine Fisheries and Sustainable Development,Ministry of Agriculture and Rural Affairs,Qingdao 266071,China;Laboratory for Marine Fisheries Science and Food Production Processes,Qingdao 266071,China;Freshwater Fisheries Science Academy of Liaoyang 111000,China;Laizhou Mingbo Aquatic Products Co.,Ltd,Laizhou 261418,China;Fujian Yiyou Aquatic Science and Technology Co.Ltd,Zhangzhou 363402,China)

机构地区：[1]中国水产科学研究院黄海水产研究所农业农村部海洋渔业与可持续发展重点实验室,山东青岛266071 [2]海洋渔业科学与食物产出过程功能实验室,山东青岛266071 [3]辽宁省淡水水产科学研究院,辽宁辽阳111000 [4]莱州明波水产有限公司,山东莱州261418 [5]福建省逸有水产科技有限公司,福建漳州363402

出　　处：《渔业科学进展》2024年第1期202-210,共9页Progress in Fishery Sciences

基　　金：中国水产科学研究院黄海水产研究所基本科研业务费(20603022020018);财政部和农业农村部:现代农业产业技术体系(CARS-49)共同资助。

摘　　要：为了解莱氏拟乌贼(Sepioteuthis lessoniana)输卵管腺的结构和功能,通过解剖学、组织切片和透射电镜技术对腺体进行解剖学及超显微结构观察。解剖结果显示,莱氏拟乌贼具有1个输卵管腺,由圆饼状近端输卵管腺和圆柱状远端输卵管腺构成,整体呈白色,靠近内壳侧具有棕黄色的色素沉着,透明瓣膜位于腺体末端。显微结果显示,输卵管腺由腺壁组织包裹,由外向内分别是外膜层和结缔组织。外膜层由柱状上皮细胞构成,结缔组织中分布少量肌肉组织和血管;腺体的外膜层和透明瓣膜的外表层中分布少量杯状细胞,瓣膜主要由肌肉组织构成。腺体内部是分泌叶瓣,在腺体中呈层状分布,叶瓣依附于腺壁组织。电镜结果显示,分泌细胞个体较大,细胞核呈圆形或椭圆形,包含大量内质网、线粒体和高尔基体等细胞器,此外,胞内分布大量黏液颗粒,细胞外连续着生纤毛。透明瓣膜主要由肌细胞构成,可见横切和纵切的肌丝以及大量的胶原纤维。研究表明,输卵管腺具备分泌功能,其分泌物形成受精卵的内层卵鞘,在受精卵孵化过程中具有重要作用。本研究首次对莱氏拟乌贼的输卵管腺进行超显微结构观察,描述了输卵管腺的外部形态构造和内部结构特征,分析并总结了该腺体的结构和功能,为头足类生殖腺体的相关研究提供一定参考。Cephalopods,which have a rapid growth rate and short life cycle,are regarded as an important marine fishing resource.Sepioteuthis lessoniana is among the most widely distributed species in the Loliginidae family in the Indo-Western Pacific Ocean.Its meat is delicious and nutritious.It is widespread in the East China Sea,South China Sea,and other marine areas in China,and it is considered an important local marine resource.In this study,the ultrastructure of the oviducal gland of S.lessoniana was investigated for the first time via anatomical dissection,tissue sectioning,and electron microscope projection.The external morphology and internal structure of the gland were clearly described,as well as the role of the oviduct gland in the reproductive activities of cephalopods.In this experiment,all the samples were collected from the open ocean,with the samples of wild S.lessoniana coming from marine areas in Fujian Province.The body surfaces of the samples were healthy and undamaged,with an average mantle length of(17.5±6.4)cm and an average body weight of(392.0±76.0)g.The samples were dissected using standard anatomical methods,and the glandular characteristics were recorded.The oviducal gland was dissected with a scalpel for tissue sectioning and preserved for electron microscope projection and observation.The experimental results showed that S.lessoniana had a single oviducal gland,which was located on the right side of its abdominal cavity.The sexually mature gland was milky white overall,with brownish-yellow pigmentation near the inner shell.The oviducal gland was enveloped in a transparent membrane,and regular gaps were visible.The gland consisted of three parts:the proximal oviducal gland,the distal oviducal gland,and the transparent valve.The proximal oviducal gland was infundibular and connected with the hyaline oviduct.The distal oviducal gland was cylindrically tapering,and a single mature egg was observed inside.The transparent valve was at the end of the gland,also known as the valve.The microstructure

关 键 词：莱氏拟乌贼 输卵管腺 显微结构 超微结构 

分 类 号：S965[农业科学—水产养殖]