养殖环境及贝源溶藻弧菌MLST分型及其毒力基因、耐药性分析  

Study on Multi-Locus Sequence Typing,Virulence Genes,and Drug Resistance of Vibrio alginolyticus from Shellfish and Culture Environment

作　　者：郑玉东 张翔 姚梦丽 谷莉 黄博闻 辛鲁生 白昌明[2] 王崇明[2] 唐小千[1] ZHENG Yudong;ZHANG Xiang;YAO Mengli;GU Li;HUANG Bowen;XIN Lusheng;BAI Changming;WANG Chongming;TANG Xiaoqian(Laboratory of Pathology and Immunology of Aquatic Animals,Ocean University of China,Qingdao 266003,China;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Laboratory for Marine Fisheries Science and Food Production Processes,Key Laboratory of Maricultural Organism Disease Control,Ministry of Agriculture and Rural Affairs,Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity,Qingdao 266071,China)

机构地区：[1]中国海洋大学水产动物病害与免疫学实验室,山东青岛266003 [2]中国水产科学研究院黄海水产研究所海洋渔业科学与食物产出过程功能实验室农业农村部海水养殖病害防治重点实验室青岛市海水养殖流行病学与生物安保重点实验室,山东青岛266071

出　　处：《渔业科学进展》2024年第1期211-223,共13页Progress in Fishery Sciences

基　　金：国家自然科学基金(32073014);青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室开放课题(2019-BH-A02);财政部和农业农村部:国家现代农业产业技术体系共同资助。

摘　　要：溶藻弧菌(Vibrio alginolyticus)是近年来贝类病害中最常见的细菌性病原之一,对贝类养殖产业的健康发展构成严重威胁。本研究旨在分析不同养殖环境水体和贝类组织中,溶藻弧菌的基因变异、毒力基因、耐药性及其分布规律。对12株溶藻弧菌分离株开展多位点序列分型(multilocus sequence typing,MLST)、毒力因子以及菌株耐药性分析,结果显示,12株溶藻弧菌的序列型(sequence typing,ST)分型互不相同,7株为Pub MLST数据库已经收录的ST型,5株因管家基因的等位基因位点变化而形成新的ST型,贝类养殖环境中的溶藻弧菌具有较高的遗传多样性。12株溶藻弧菌都携带tlh、fur和collagenase三种毒力基因,但均未检测到tdh、trh、toxR和tcpA毒力基因。溶藻弧菌携带毒力因子的种类和数量受地区分布等因素的影响。不同来源的溶藻弧菌均具有多重耐药特征,对青霉素和氨苄西林产生抗性。本研究表明,贝类养殖环境中的溶藻弧菌具有种群复杂、遗传多样性高的特点;不同来源菌株在毒力基因携带和耐药性方面存在较大差异。本研究通过探究不同区域内不同来源的溶藻弧菌遗传变异及耐药性差异,对贝源溶藻弧菌的有效防控提供一定理论参考。China is the most productive country in the world in terms of shellfish farming,with seawater shellfish occupying a dominant position in China´s shellfish farming industry.In the past two decades,China´s marine shellfish culture has been enriched in terms of species and culture methods.Moreover,its scale has expanded,with annual production remaining above 10 million tons and the total economic output exceeding 220 billion yuan.However,the economic losses caused by epidemic diseases in the shellfish aquaculture industry are also increasing annually,exceeding 10 billion yuan in 2021.These diseases have become some of the primary limiting factors for the healthy development of the shellfish aquaculture industry.Epidemiological surveys in recent years have shown that vibriosis is the most prevalent bacterial disease and the leading cause of mass mortality in shellfish farming.Vibrio alginolyticus is one of the most common Vibrio pathogens in shellfish diseases,posing a grave threat to the healthy development of the shellfish farming industry.However,effective methods for preventing and controlling V.alginolyticus are still lacking.The pathogenicity of V.alginolyticus is frequently closely related to its virulence factors and biological characteristics,and it is unclear how the virulence factors and biological characteristics of V.alginolyticus vary according to different sources and regions.Therefore,in this study,we aimed to gain a deeper understanding of the pathogenicity and scientific control of V.alginolyticus by analyzing the genetic variation and distribution patterns of V.alginolyticus in different aquaculture environments and shellfish tissues.The study was conducted to observe the external morphological characteristics and conduct 16S identification of twelve V.alginolyticus isolates collected from four regions:Qingdao,Weifang,Weihai,and Yantai.These were isolated and purified in TCBS selective medium;MLST typing of the strains by four housekeeping genes;the distribution of thirteen Vibrio virulence genes

关 键 词：溶藻弧菌 MLST 毒力基因 耐药性分析 

分 类 号：S944.4[农业科学—水产养殖]