肿瘤细胞3D无支架培养技术研究进展及其在药效评价中应用  

Research progress in 3D scaffold-free culture of tumor cell and its application in pharmacodynamic evaluation

作　　者：陈艳阁 汪海峰 CHEN Yan’ge;WANG Haifeng(College of Chemical Engineering,Shenyang University of Chemical Technology,Shenyang 110142,China)

机构地区：[1]沈阳化工大学化学工程学院,沈阳110142

出　　处：《上海医药》2024年第1期75-79,共5页Shanghai Medical & Pharmaceutical Journal

基　　金：辽宁省教育厅科学研究经费项目青年科技人才“育苗”项目(LQ2020021)。

摘　　要：作为三维(3D)细胞培养技术之一的3D无支架培养技术,其广泛应用于肿瘤3D细胞模型的构建之中。了解当前肿瘤细胞3D无支架培养技术的应用情况,以及肿瘤3D细胞模型的构建成果对于抗癌药物的研发与评价很重要。本文介绍了构建肿瘤3D细胞模型的超低吸附着板培养法、悬滴培养法、磁悬浮培养法和旋转式细胞培养系统以及它们的局限性,并简述了肿瘤3D细胞模型用于药效评价的新进展,以期为肿瘤3D细胞模型的构建及抗癌药的药效评价提供参考。As one of the three-dimensional(3D)cell culture technologies,the 3D scaffold-free culture technology is widely used in the construction of tumor 3D cell models.It is important to understand the current application of tumor cell 3D scaffold-free culture techniques and the results of tumor 3D cell model construction for the development and evaluation of anti-tumor drugs.This article introduces the ultra-low attachment plates culture,hanging drop technique,magnetic levitation culture and rotary cell culture system for constructing a 3D cell model of tumors and their limitations,and outlines some new progress of the application of 3D cell models in the pharmacodynamic evaluation so as to provide reference for the construction of 3D cell models of tumors and the efficacy evaluation of anticancer drugs.

关 键 词：3D肿瘤细胞模型 无支架细胞培养技术 药效评价 

分 类 号：R965[医药卫生—药理学]