纯钛表面氧化石墨烯涂层的制备及生物学性能研究  

Preparation and Biological Properties of Graphene Oxide Coating on Pure Titanium Surface

作　　者：文超举 刘春影 舒佳玉 陆婧 王欢 董强 徐星星 WEN Chaoju;LIU Chunying;SHU Jiayu;LU Jing;WANG Huan;DONG Qiang;XU Xingxing(School of Stomatology,Guizhou Medical University,Guiyang 550004,China;Affiliated Stomatological Hospital,Guizhou Medical University,Guiyang 550004,China)

机构地区：[1]贵州医科大学口腔医学院,贵阳550004 [2]贵州医科大学附属口腔医院,贵阳550004

出　　处：《有色金属工程》2024年第2期1-8,共8页Nonferrous Metals Engineering

基　　金：国家自然科学基金资助项目(81860192);贵州医科大学附属口腔医院/口腔医学院科研基金(GYKQKY-2020-05)。

摘　　要：利用3-氨丙基三乙氧基硅烷在纯钛表面制备氧化石墨烯涂层,扫描电镜、X射线能谱仪、拉曼光谱、X射线光电子能谱技术对涂层的形貌结构和成分进行表征检测;之后通过体外细胞毒性实验、急性溶血实验和SBF模拟体液浸泡实验评估不同处理组钛片的生物学活性。结果表明:体外小鼠前成骨细胞毒性实验、急性溶血实验初步证实复合涂层具有良好的生物相容性;SBF模拟体液浸泡实验表明氧化石墨烯涂层能提高纯钛表面沉积羟基磷灰石的能力。Graphene oxide coating was prepared on the surface of pure titanium via using 3-aminopropyltriethoxysilane.Scanning electron microscopy,raman spectroscopy,X-ray energy spectrometer and X-ray photoelectron spectroscopy were adopted to characterize the morphology,structure and composition of the coating.The biological activity of titanium sheets in different treatment groups was evaluated via in vitro cytotoxicity test,acute hemolysis test and SBF simulated body fluid immersion test.The results showed that the graphene oxide coating was prepared on the surface of pure titanium by silane coupling agent.The in vitro cytotoxicity test and acute hemolysis test preliminarily confirmed that the composite coating had good biocompatibility.The SBF simulated body fluid immersion experiment showed that the graphene oxide coating could improve the ability of hydroxyapatite deposition on the surface of pure titanium.

关 键 词：纯钛 硅烷偶联剂 氧化石墨烯 生物相容性 细胞培养 

分 类 号：TG174.4[金属学及工艺—金属表面处理]