基于UPLC-Q-Exactive-Orbitrap-MS的胃康灵胶囊化学成分分析  

Chemical components of Weikangling capsules by UPLC-Q-Exactive-Orbitrap-MS

作　　者：刘润花 谭征[1] 杨畅 杨子烨 关晶[1] 张建民[1] LIU Run-hua;TAN Zheng;YANG Chang;YANG Zi-ye;GUAN Jing;ZHANG Jian-min(Department of Pharmacy,Children's Hospital Affiliated to Capital Institute of Pediatrics,Beijing 100020)

机构地区：[1]首都儿科研究所附属儿童医院制剂室,北京100020

出　　处：《中南药学》2024年第1期56-64,共9页Central South Pharmacy

基　　金：十三五重大新药创制科技重大专项(No.2018ZX09721003-009-005)。

摘　　要：目的 建立超高效液相色谱串联四极杆静电场轨道阱质谱(UPLC-Q-Exactive-OrbitrapMS)法准确、快速定性鉴别胃康灵胶囊中的化学成分。方法 采用Waters ACQUITY UPLC HSS T3 C18色谱柱(1.7μm,2.1 mm×150 mm),以0.1%甲酸水溶液(A)-乙腈(B)为流动相进行梯度洗脱,流速为0.3 mL·min^(-1),柱温40℃,进样量2μL,电喷雾电离源(ESI)在正、负离子两种模式下采集数据,扫描方式为全扫描/数据依赖二级扫描(Full MS/dd-MS~2)。结果 在胃康灵胶囊水提液中共鉴定出142个化学成分,包括三萜类58个、生物碱类32个、单萜类17个、黄酮类12个、多酚类10个、有机酸类6个,菲类3个、香豆素类1个、木脂素类1个、苯并吡喃类1个和氮苷类1个。结论 UPLC-Q-Exactive-Orbitrap-MS技术能够快速有效地对胃康灵胶囊中化学成分进行鉴定,为进一步明确其药效物质提供参考。Objective To qualitatively analyze the chemical components of Weikangling capsules based on UPLC-Q-Exactive-Orbitrap-MS.Methods A Waters ACQUITY UPLC HSS T3 C18 column(1.7μm,2.1 mm×150 mm) was used for the gradient elution with 0.1%formic acid aqueous solution(A)-acetonitrile (B) as the mobile phase,with a flow rate of 0.3 mL·min^(-1),column temperature of 40℃,and injection volume of 2μL.The data were collected in both positive and negative ion modes by electrospray ionization source.The scanning mode was full scan/data dependent secondary scan (Full MS/dd-MS~2).Results Totally 142 chemical compounds,including 58 triterpenoids,32alkaloids,17 monoterpenoids,12 flavonoids,10 polyphenols,6 organic acids,3 phenanthrenes,1coumarin,1 lignan,1 chromene and 1 nitrogen glycoside were identified in the water extraction of Weikangling capsules.Conclusion UPLC-Q-Exactive-Orbitrap-MS can quickly and effectively identify the chemical compounds in Weikangling capsules to clarify the effective substances of Weikangling capsules.

关 键 词：胃康灵胶囊 化学成分分析 超高效液相色谱串联四极杆静电场轨道阱质谱 

分 类 号：R284.1[医药卫生—中药学]