静脉注射吴茱萸碱-甘草酸纳米胶束后吴茱萸碱在大鼠体内药代动力学研究  

In vivo pharmacokinetics of evodiamine in rats after intravenous injection of evodiamine-glycyrrhizic acid micelles

作　　者：邓英光[1] 银雪艳 郭秀彩[1] 张紫萍[1] 李峰[1] DENG Ying-guang;YIN Xue-yan;GUO Xiu-cai;ZHANG Zi-ping;LI Feng(Guangzhou Twelfth People’s Hospital,Guangzhou 510620)

机构地区：[1]广州市第十二人民医院,广州510620

出　　处：《中南药学》2024年第1期141-144,共4页Central South Pharmacy

基　　金：广东省中医药局中医药科研项目(No.20222154)。

摘　　要：目的 建立高效液相色谱法(HPLC)测定大鼠血浆中的吴茱萸碱,研究大鼠尾静脉注射吴茱萸碱-甘草酸纳米胶束后的药代动力学情况。方法 大鼠尾静脉注射吴茱萸碱-甘草酸纳米胶束和吴茱萸碱溶液后,采用HPLC测定大鼠血浆中吴茱萸碱的含量,绘制血药浓度-时间曲线,以DAS 2.0软件分析处理药代动力学参数。结果 吴茱萸碱-甘草酸纳米胶束和吴茱萸溶液的Cmax分别为(1.61±0.19)和(0.92±0.11)μg·mL^(-1),AUC_(0~t)分别为(4.76±1.41)和(2.07±0.22)mg·L^(-1)·h,CL分别为(0.67±0.21)和(1.38±0.14)L·h^(-1)·kg^(-1),吴茱萸碱-甘草酸纳米胶束的C_(max)、AUC_(0~t)较吴茱萸碱溶液提高了1.75、2.30倍,CL是吴茱萸碱溶液的0.48倍,C_(max)、AUC_(0~t)和CL差异均有统计学意义(P <0.05)。结论 将吴茱萸碱制备为吴茱萸碱-甘草酸纳米胶束能延长吴茱萸碱在体内的作用时间,延缓吴茱萸碱在体内的代谢。Objective To establish an HPLC method to determine evodiamine in rat plasma and to study the intravenous pharmacokinetics of evodiamine-glycyrrhizic acid micelles in rats.Methods The rats were administerted evodiamine-glycyrrhizic acid micelles and evodiamine solution,respectively.The content of evodiamine in the rat plasma was determined by HPLC,and the pharmacokinetic parameters were analyzed by DAS 2.0 software.Results The Cmax of evodiamine-glycyrrhizic acid micelles and evodiamine solution was(1.61±0.19) and(0.92±0.11) μg·mL^(-1);AUC_(0 ~ t) was(4.76±1.41) and(2.07±0.22) mg·L^(-1)·h;CL was(0.67±0.21) and(1.38±0.14) L·h^(-1)·kg^(-1),respectively.The C_(max) and AUC_(0 ~ t) were 1.75 and 2.30 times higher than those of evodiamine solution,while CL was 0.48times higher than that of evodiamine solution.The difference in C_(max),AUC_(0 ~ t) and CL was significant(P < 0.05).Conclusion The conversion of evodiamine into micelles with glycyrrhizic acid may prolong the duration and metabolic processes of evodiamine in vivo.

关 键 词：吴茱萸碱 甘草酸 胶束 药代动力学 

分 类 号：R944[医药卫生—药剂学]