胸腹水液基细胞学检测联合胸腹水沉淀物琼脂石蜡双包埋切片法在病理检测的价值  

The Value of Combining Liquid-Based Cytological Detection of Pleural and Ascitic Fluid with Agar Paraffin Double Embedding Section Method in Pathological Detection

作　　者：田莉 吴阳春 TIAN Li;WU Yang-chun(Department of Pathology,Urumqi Hospital of Traditional Chinese Medicine,Urumqi Xinjiang 830000,China)

机构地区：[1]乌鲁木齐市中医医院病理科,新疆乌鲁木齐830000

出　　处：《中华养生保健》2024年第3期81-84,共4页CHINESE HEALTH CARE

摘　　要：目的探讨与分析胸腹水液基细胞学检测联合胸腹水沉淀物琼脂石蜡双包埋切片法在病理检测的价值。方法选取2019年3月—2022年9月在乌鲁木齐市中医医院诊治的92例胸腹水患者作为研究对象,所有患者都给予胸腹水液基细胞学检测联合胸腹水沉淀物琼脂石蜡双包埋切片法,都给予病理活检,判断诊断的价值。结果在92例患者中,胸腹水液基细胞学检测判断为恶性肿瘤54例,良性肿瘤38例,胸腹水液基细胞学检测诊断恶性肿瘤的敏感度与特异度分别为85.48%、96.67%。胸腹水沉淀物琼脂石蜡双包埋切片检测判断为恶性肿瘤55例,良性肿瘤37例,胸腹水沉淀物琼脂石蜡双包埋切片检测诊断恶性肿瘤的敏感性与特异性分别为88.71%、100.00%。胸腹水液基细胞学检测联合胸腹水沉淀物琼脂石蜡双包埋切片检测判断为恶性肿瘤61例,良性肿瘤31例,胸腹水液基细胞学检测联合胸腹水沉淀物琼脂石蜡双包埋切片检测诊断恶性肿瘤的敏感度与特异度分别为98.39%、100.00%。恶性肿瘤患者的人表皮生长因子受体2、突触素、嗜铬素A表达阳性率分别为40.32%、41.94%、43.55%,与良性肿瘤患者的10.00%、13.33%、10.00%相比有显著提高,差异有统计学意义(P<0.05)。胸腹水液基细胞学检测联合胸腹水沉淀物琼脂石蜡双包埋切片法对肿瘤良恶性的诊断敏感性高于单项检测,差异有统计学意义(P<0.05),不同检测方法的诊断特异性比较,差异无统计学意义(P>0.05)。ROC曲线分析显示,胸腹水液基细胞学检测联合胸腹水沉淀物琼脂石蜡双包埋切片法对肿瘤良恶性的诊断曲线下面积为0.883。结论胸腹水液基细胞学检测联合胸腹水沉淀物琼脂石蜡双包埋切片法在病理检测中的应用能提高诊断敏感度,还可保持非常高的诊断特异度,对患者的原发肿瘤良恶性判定具有很好的价值。Objective To explore and analysis the values of liquid based cytology combined with agar paraffin double embedding section method for pathological detection of pleural and ascitic fluid sediment.Methods From March 2019 to September 2022,92 cases of patients with pleural and ascitic fluid who were diagnosed and treated at a certain hospital were selected as the research subjects.All patients were underwent liquid based cytology testing of pleural and ascitic fluid combined with agar paraffin double embedding section method for pleural and ascitic fluid sediment.Pathological biopsy were also performed to determine the diagnostic value.Results There were 54 cases were diagnosed as malignant tumors and 38 cases were diagnosed as benign tumors by pleural and ascitic fluid based cytology in the 92 cases.The sensitivity and specificity of pleural and ascitic fluid based cytology in diagnosing malignant tumors were 85.48%and 96.67%,respectively.The sensitivity and specificity of agarose paraffin double embedding sections in the diagnosis of malignant tumors were 88.71%and 100.00%,respectively,and 56 cases of malignant tumors and 36 cases of benign tumors were detected by the method.The sensitivity and specificity of agarose paraffin double embedding section detection of pleural and ascitic fluid sediment in diagnosing malignant tumors were 98.39%and 100.00%,respectively,and 61 cases of malignant tumors and 31 cases of benign tumors were detected by the method.The positive rates of human epidermal growth factor receptor 2,synaptophysin,and chromaffin A expression in malignant tumor patients were 40.32%,41.94%,and 43.55%,respectively,which were significantly higher than those in benign tumor patients(10.00%,13.33%,10.00%,P<0.05).The sensitivity of the combination of liquid based cytology detection of pleural and ascitic fluid and agar paraffin double embedding section method for the diagnosis of benign and malignant tumors is higher than that of single detection(P<0.05),and there is no significant difference in the diagnos

关 键 词：胸腹水 液基细胞学 胸腹水沉淀物 琼脂石蜡双包埋切片 恶性肿瘤 良性肿瘤 

分 类 号：R446.1[医药卫生—诊断学]