利用SNP芯片构建玉米品种新科910和新科891 DNA指纹数据库  

Construction of DNA Fingerprinting Databases for Maize Varieties Xinke910 and Xinke891 Based on SNP Chip

作　　者：孙佩[1] 张培风 周联东[1] 任帅 张玉红[1] 李合顺[1] 王学军[1] SUN Pei;ZHANG Peifeng;ZHOU Liandong;REN Shuai;ZHANG Yuhong;LI Heshun;WANG Xuejun(Xinxiang Academy of Agricultural Sciences,,Xinxiang He'nan 453000,China)

机构地区：[1]新乡市农业科学院,河南新乡453000

出　　处：《耕作与栽培》2023年第6期1-3,共3页Tillage and Cultivation

基　　金：河南省重大科技专项(201300111100);河南省农业良种联合攻关项目(2022010201)。

摘　　要：以玉米品种新科910和新科891,玉米自交系K381、H865、S155和PHA458为试验材料,利用玉米10K SNP芯片进行基因分型,选择双亲和杂交种SNP相同的纯合型标记构建SNP指纹数据库,新科910共有3720个纯合型标记,新科891共有2670个纯合型标记,可有效鉴定该品种的真实性和特异性。In this study,maize varieties Xinke910 and Xinke891,as well as the maize inbred lines K381,H865,S155,and PHA458,were used as experimental materials.Using the maize 10K SNP chip for genotyping,homozygous markers with the same SNPs as the parents and hybrid varieties were selected to construct an SNP fingerprint database.Xinke910 had a total of 3720 homozygous markers,while Xinke891 had 2670 homozygous markers.This database could effectively identify the authenticity and specificity of these maize varieties.

关 键 词：玉米 SNP标记 新科910 新科891 指纹数据库 

分 类 号：S513[农业科学—作物学]