基于显微特征颜色量化评价判别栀子与焦栀子  

Distinguishing Between Zhizi(Gardeniae Fructus) and Jiaozhizi(Gardeniae Fructus Praeparatus) Based on Quantitative Evaluation of Microscopic Characteristic Color

作　　者：王玲[1] 张学兰[1,2] 李慧芬 孔庆悦[1] 陈鑫凤[1] 刘阳阳 杨雨思 WANG Ling;ZHANG Xuean;LI Huifen;KONG Qingyue;CHEN Xinfeng;LIU Yangyang;YANG Yusi(Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Shandong Province University Collaborative Innovation Center of Traditional Chinese Medicine Quality Control and Construction of Whole Industry Chain,Jinan 250355,China)

机构地区：[1]山东中医药大学,山东济南250355 [2]山东省高校中药质量控制与全产业链建设协同创新中心,山东济南250355

出　　处：《山东中医药大学学报》2024年第1期100-105,共6页Journal of Shandong University of Traditional Chinese Medicine

基　　金：国家重点研发计划项目(编号:2018YFC1707204,2018YFC1707002);山东省高校中药质量控制与全产业链建设协同创新中心资助项目(编号:CYLXTCX2021-12);国家中医药管理局中药炮制技术传承地项目(批文号:国中医药科技中药[2022]59号);山东省中医药科技发展计划项目(编号:2019-0955)。

摘　　要：目的:建立基于显微特征颜色量化评价的栀子与焦栀子判别方法。方法:采用显微成像技术和显微特征颜色提取软件测定栀子与焦栀子显微特征颜色,利用Kruska-WallisH秩和检验、Fisher判别分析法分析栀子与焦栀子显微特征颜色差异,建立判别函数。结果:栀子与焦栀子显微特征(内果皮石细胞、内果皮纤维、种皮石细胞)的亮度值(L^(*))、红绿色值(a^(*))、黄蓝色值(b^(*))和总色度值(E_(ab)^(*))均有显著差异(P<0.01)。以种皮石细胞颜色对二者进行判别,其判别函数为y=0.678×L^(*)+0.384×a^(*)-0.576×b^(*)-9.322,y>0为栀子,y<0为焦栀子。结论:通过显微特征颜色量化评价,实现了栀子与焦栀子的有效判别,为栀子生、制饮片的判别和质量评价提供了新方法。Objective:To establish a method to distinguish Zhizi(Gardeniae Fructus)and Jiaozhizi(Gardeniae Fructus Praeparatus)based on quantitative evalua-tion of microscopic characteristic color.Methods:The microscopic imaging technology and the micro-scopic characteristics color extraction software were used to measure the microscopic characteristic color.Kruska-Wallis H test and Fisher discriminant analysis method were used to analyze the micro-scopic characteristic color differences between Zhizi and Jiaozhizi,and a discriminant function was established.Results:There were significant differ-ences in the lightness value(L^(*)),red-green value(a^(*)),yellow-blue value(b^(*)),total chromaticity values(E^(*)_(ab))of microscopic characteristics(endocarp cell,endocarp fiber,seed stone cell)between Zhizi and Jiaozhizi(P<0.01).Zhizi and Jiaozhizi were distinguished by the color of seed stone cells and the discriminant func-tion of microscopic characteristic color:y=0.678×L^(*)+0.384×a^(*)-0.576×b^(*)-9.322,y>0 corresponding to Zhizi,y<0 corresponding to Jiaozhizi.Conclusions:The effective discrimination of Zhizi and Jiaozhizi is realized through the quantitative evaluation of microscopic characteristic color,which provides a new method for the discrimination and quality evaluation of raw and processed decoction pieces of Zhizi.

关 键 词：栀子 焦栀子 显微特征颜色 量化评价 显微成像技术 FISHER判别分析 

分 类 号：R282.740.3[医药卫生—中药学]