黔南州和贵阳市幽门螺杆菌临床菌株的耐药性、基因多样性及其与疾病的关系  

Drug resistance and genetic diversity of clinical strains of Helicobacter pylori in the Qiannan Prefecture and Guiyang City,and their relationships with diseases

作　　者：张远远 潘科 糜孟衡 管玉祝 陆秋丹 郑娟 张晋 王天姝 刘琦 陈峥宏 ZHANG Yuan-yuan;PAN Ke;MI Meng-heng;GUAN Yu-zhu;LU Qiu-dan;ZHENG Juan;ZHANG Jin;WANG Tian-shu;LIU Qi;CHEN Zheng-hong(Department of Gastroenterology,Affiliated Hospital of Guizhou Medical University,Guiyang 550004,China;Department of Microbiology,School of Basic Medicine,Guizhou Medical University/Key Laboratory of Pathogenic Biology Characteristics in Guizhou Universities,Guiyang 561113,China;People’s Hospital of Qiannan Prefecture,Qiannan 558000,China)

机构地区：[1]贵州医科大学附属医院消化内科,贵阳550004 [2]贵州医科大学基础医学院微生物教研室/贵州省普通高校病原生物学特色重点实验室,贵阳561113 [3]黔南州人民医院,黔南558000

出　　处：《中国人兽共患病学报》2024年第1期46-55,共10页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(No.81860353);贵州省自然科学基金(No.19NSP022[2020]1Y341);贵州省科技计划项目(No.ZK[2022]368);贵州省教育厅滚动支持省属高校科研平台团队项目(No.[2022]019)联合资助。

摘　　要：目的了解贵州省贵阳市和黔南州少数民族地区幽门螺杆菌的耐药性、基因多样性及其与临床疾病的关系。方法通过内窥镜采集样本分离培养幽门螺杆菌,测定耐药性和基因型特征,比较不同地区、疾病类型的差异,分析幽门螺杆菌结构特点及黔南州幽门螺杆菌不同菌型混合感染情况。结果两地区cagA可变区EPYIA分型构成比差异有统计学意义(P=0.012),vacA基因型构成比差异有统计学意义(P=0.000);MLST方法得到两地区幽门螺杆菌菌株94.6%(53/56)新的序列型。黔南州幽门螺杆菌不同菌型菌株混合感染率为44.4%,不同临床疾病的幽门螺杆菌不同菌型混合感染构成比差异无统计学意义(P=0.349)。结论黔南州和贵阳市两地区幽门螺杆菌cagA可变区EPIYA分型和vacA基因型构成比存在差异。To understand Helicobacter pylori’s drug resistance,genetic diversity,and relationship with clinical diseases in the Guiyang and Qiannan minority areas of Guizhou Province,we collected samples through endoscopy,and isolated and cultured H.pylori.The drug resistance and genotype characteristics were determined.The differences in different regions and disease types were compared,and the structural characteristics of H.pylori and mixed infections with different strains of H.pylori in Qiannan Prefecture were analyzed.A difference in the composition ratio of EPYIA typing in the cagA variable region was observed between the two areas(P=0.012),and the com position ratio of the vacA genotype differed(P=0.000).A total of 94.6%(53/56)new sequences of H.pylori strains from two regions were obtained by MLST.The rate of infection by H.pylori mixed with different strains was 44.4%in Qiannan Prefecture,and no significant difference was observed in the composition of H.pylori mixed infections among patients with different clinical diseases(P=0.349).Differences in EPIYA typing and the vacA genotype composition ratio in the cagA variable region of H.pylori were observed between the Qiannan Prefecture and Guiyang City.

关 键 词：幽门螺杆菌 黔南州 贵阳市 基因多态性 混合感染 

分 类 号：R378.2[医药卫生—病原生物学]