江苏省某监测点家畜来源肠致病性大肠埃希菌分离株分子特征分析  

Molecular characteristics of enteropathogenic Escherichia coli isolates from livestock at a monitoring point in Jiangsu Province

作　　者：孔筱筱 韩悦 周璐[1] 程晓庆 董晨[1] KONG Xiao-xiao;HAN Yue;ZHOU Lu;CHENG Xiao-qing;DONG Chen(Jiangsu Center for Disease Control and Prevention,Key Laboratory of Intestinal Pathogenic Microorganisms,National Health Commission,Nanjing 210009,China;Hangzhou Yuanda Biopharmaceutical Co.,Ltd,Hangzhou 310000,China)

机构地区：[1]国家卫生健康委员会肠道病原微生物重点实验室,江苏省疾病预防控制中心,南京210009 [2]杭州远大生物制药有限公司,杭州310000

出　　处：《中国人兽共患病学报》2024年第1期56-61,共6页Chinese Journal of Zoonoses

基　　金：十四五流行病学重点学科(No.ZDXK202250)。

摘　　要：目的分析江苏省某监测点家畜来源肠致病性大肠埃希菌分离株分子特征,进而评估其潜在的致病性,为感染性腹泻的监测与防控提供依据。方法采用全基因组测序技术对江苏省某监测点分离的家畜来源的37株肠致病性大肠埃希菌(EPEC)菌株进行特征性分析。结果本地家畜来源的EPEC均为非典型EPEC(aEPEC),其血清型多样,携带多种与腹泻相关的毒力基因,有9种ST型且具有区域性流行特征,eae基因包含有5种亚型,β1为优势亚型,其在腹泻病人中也最为常见。结论aEPEC对公众健康构成潜在威胁,应在感染性腹泻的监测工作中,加强对家畜粪便及养殖环境的监测。This study was aimed at analyzing the molecular characteristics of enteropathogenic Escherichia coli(E.coli)strains isolated from domestic animals at a surveillance site in Jiangsu province and evaluating their potential pathogenicity,to provide evidence supporting the surveillance,prevention,and control of infectious diarrhea.Thirty-seven EPEC strains isolated from domestic animals at this surveillance site were characterized by whole genome sequencing.All EPEC strains isolated from local livestock were aEPEC,which has a variety of serotypes and carries a variety of virulence genes associated with diarrhea.Nine ST types with regional epidemic characteristics were identified.Five eae gene subtypes were found,among whichβ1 was dominant and was also the most common strain in patients with diarrhea.According to analysis of the characteristics of 37 EPEC strains,all EPEC strains from local livestock were aEPEC,thus posing a potential threat to public health.Monitoring of livestock feces and the breeding environment must be strengthened in the surveillance of infectious diarrhea.

关 键 词：肠致病性大肠埃希菌 感染性腹泻 分子特征分析 

分 类 号：R378.21[医药卫生—病原生物学]