1例输入性类鼻疽的病原学诊断及溯源分析  

Etiological diagnosis and molecular tracing analysis in a case of imported melioidosis

作　　者：杨红霞[1] 王春雨[2] 王洋[1] 郝瑞娥[1] 赵启玉[3] 郑霄[4] YANG Hong-xia;WANG Chun-yu;WANG Yang;HAO Rui-e;ZHAO Qi-yu;ZHENG Xiao(Shanxi Provincial Key laboratory for Major Infectious Disease Response,Shanxi Provincial Center for Disease Control and Prevention,Taiyuan 030012,China;Shanxi Provincial People’s Hospital,Taiyuan 030012,China;Taiyuan Municipal Center for Disease Control and Prevention,Taiyuan 030032,China;State Key laboratory for Infectious Disease Prevention and Control,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]山西省疾病预防控制中心,重点传染性疾病预防与诊治山西省重点实验室,太原030012 [2]山西省人民医院,太原030012 [3]太原市疾病预防控制中心,太原030032 [4]中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,北京102206

出　　处：《中国人兽共患病学报》2024年第1期76-81,共6页Chinese Journal of Zoonoses

摘　　要：目的对1例输入性类鼻疽患者进行病原学诊断及溯源分析,为山西省类鼻疽防控提供科学依据。方法患者血培养阳性,分离菌株鉴定为泰国伯克霍尔德菌。采用生化试验及微生物质谱仪进行菌株鉴定,核酸提取后进行全基因组测序,分析MLST型和耐药基因携带情况,同时构建系统进化树。结果此次分离株经生化试验及微生物质谱仪检测均鉴定为类鼻疽伯克霍尔德菌,MLST型为ST366,全基因测序分析与海南省3个分离株进化亲缘关系较近,同源性较高。结论此例类鼻疽病例经分子溯源分析及流行病学调查确定为海南省输入,提示医疗机构及疾控部门应加强对类鼻疽的认识,提高诊疗能力。We aimed to identify the infectious source of a case of melioidosis,to provide evidence for the prevention and control of melioidosis in Shanxi Province,China.The patient developed repeated fever,fatigue,diarrhea,and other symptoms after being caught in the rain while traveling in Hainan Province.The blood culture was positive,and the bacterial strain was identified as Burkholderia thayensis and sent to the provincial Center for Disease Control and Prevention for further evaluation.MALDI-TOF MS and biochemical identification were used to identify the strain,whole genome sequencing was performed after nucleic acid extraction,MLST type and drug-resistance genes were analyzed,and a phylogenetic tree was constructed.The isolated strain was identified as Burkholderia pseudomallei by MALDI-TOF MS and biochemistry,and the MLST type was 366.The whole gene sequencing analysis indicated a close evolutionary relationship with the three isolates in Hainan Province,with high homology.This case of melioidosis was indeed imported from Hainan Province,according to molecular tracing analysis and epidemiological investigation,thus suggesting that medical institutions and disease control departments should strengthen understanding of melioidosis,and improve the diagnosis and treatment ability.

关 键 词：类鼻疽 类鼻疽伯克霍尔德菌 MLST分型 全基因测序 分子溯源 

分 类 号：R378.63[医药卫生—病原生物学]