2株猪繁殖与呼吸综合征病毒海南株的ORF5基因分析  

ORF5 gene analysis of two porcine reproductive and respiratory syndrome virus strains from hainan province

作　　者：马佳镁 钟植文 范悦轩 黄春媛 郑佳馨 曹芳芳 王金泉[1] 刘光亮 曹宗喜 MA Jiamei;ZHONG Zhiwen;FAN Yuexuan;HUANG Chunyuan;ZHENG Jiaxin;CAO Fangfang;WANG Jinquan;LIU Guangliang;CAO Zongxi(College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi 830052,China;Institute of Animal Husbandry and Veterinary Medicine,Hainan Academy of Agricultural Sciences/Hainan Provincial Key Laboratory of Tropical Animal Breeding and Epidemic Research in Hanan Province/Haikou Observation Experimental Station of National Data Center of Animal Health,Haikou 571100,China;Guangdong Winsun Bio-Pharmaceutical Co.,Ltd.,Guangzhou 511536,China;Hainan Provincial Academician Innovation Center,Institute of Animal Husbandry and Veterinary Medicine,Hainan Academy of Agricultural Sciences,Haikou 571100,China)

机构地区：[1]新疆农业大学动物医学学院,乌鲁木齐830052 [2]海南省农业科学院畜牧兽医研究所/海南省热带动物繁育与疫病研究重点实验室/国家动物疫病数据中心海口观测实验点,海口571100 [3]广东永顺生物制药股份有限公司,广州511536 [4]海南省农业科学院畜牧兽医研究所海南省院士团队创新中心,海口571100

出　　处：《新疆农业科学》2023年第12期3049-3056,共8页Xinjiang Agricultural Sciences

基　　金：海南省省属科研院所技术创新专项(jscx202001);国家自然科学基金项目(32060796);家畜疫病病原生物学国家重点实验室开放基金(SKLVEB2020KFKT021);海南省农业科学院院本级科研项目(HAAS2022PT0205);国家重点研发计划(2023YFC3404302)。

摘　　要：【目的】鉴定我国海南省新发现的2株猪繁殖与呼吸综合征病毒株HNHK1-2021和HNLG1-2021的进化关系。【方法】采用PCR方法对ORF5基因进行扩增和序列测定,利用MegAlign软件进行多重序列比对,利用MEGA X软件邻接法构建进化树。【结果】PRRSV HNHK1-2021和HNLG1-2021毒株核苷酸相似性为82.9%,推导氨基酸相似性为82.6%。HNHK1-2021毒株和NADC30类PRRSV、QYYZ类PRRSV、VR-2332类PRRSV以及JXA1类PRRSV序列比对,80.8%~82.8%、90.2%~92.0%、82.1%~83.4%、81.9%~84.4%,推导氨基酸相似性分别为82.1%~84.6%、91.5%~94.5%、80.1%~83.1%、82.6%~84.6%;HNLG1-2021毒株和NADC30类PRRSV、QYYZ类PRRSV、VR2332类PRRSV以及JXA1类PRRSV序列比对,核苷酸相似性分别为83.7%~84.6%、81.8%~83.6%、97.7%~99.5%、85.6%~90.4%,推导氨基酸相似性为83.6%~85.1%、81.1%~82.6%、96.0%~98.5%,86.1%~90.5%。【结论】2株病毒株均属于北美型PRRSV,HNHK1-2021毒株属于QYYZ类PRRSV(谱系3)。HNLG1-2021毒株属于VR-2332类PRRSV(谱系5)。【Objective】This project aims to identify the evolutionary relationship of two newly discovered porcine reproductive and respiratory syndrome virus strains HNHK1-2021 and HNLG1-2021 in Hainan,China.【Methods】The ORF5 gene was amplified and sequenced by PCR method.Multiple sequence alignment was performed using MegAlign software,and phylogenetic tree was constructed using MEGA X software neighbor joining method.【Results】The nucleotide similarity of PRRSV HNHK1-2021 and HNLG1-2021 strains was 82.9%,and the deduced amino acid similarity was 82.6%.HNHK1-2021 strain and NADC30-like PRRSV,QYYZ-like PRRSV,VR2332-like PRRSV and JXA1-like PRRSV sequence alignment:80.8%-82.8%,90.2%-92.0%,82.1%-83.4%,81.9%-84.4%,deducing the amino acid similarity was 82.1%-84.6%,91.5%-94.5%,80.1%-83.1%,82.6%-84.6%;HNLG1-2021 strain and NADC30 PRRSV,QYYZ PRRSV,VR2332 PRRSV and JXA1 PRRSV sequence alignment,the nucleotide similarity of which was 83.7%-84.6%、81.8%-83.6%,97.7%-99.5%,85.6%-90.4%,the deduced amino acid similarity was 83.6%-85.1%,81.1%-82.6%,96%-98.5%,86.1%-90.5%.【Conclusion】The two virus strains belong to the North American type PRRSV,and the HNHK1-2021 strain belongs to the QYYZ class PRRSV(lineage 3).The HNLG1-2021 strain belongs to the VR-2332 class of PRRSVs(lineage 5).

关 键 词：猪繁殖与呼吸综合征病毒 ORF5基因 多重序列比对 同源性 

分 类 号：S855.2[农业科学—临床兽医学]