中度嗜盐菌Halomonas salifodinae N35-6产四氢嘧啶的培养基优化  

Optimization of medium for ectoine production by Halomonas salifodinae N35-6,a moderately halophilic bacterium

作　　者：林青[1] 秦新政[1] 高雁[1] 曾军[1] 娄恺[1] 时红玲 霍向东[1] LIN Qing;Qin Xinzheng;GAO Yan;ZENG Jun;LOU Kai;SHI Hongling;HUO Xiangdong(Institute of Microbiology,Xinjiang Academy of Agricultural Sciences/Xinjiang Laboratory of Special Enviromental Microbiology,Urumqi 830091,China)

机构地区：[1]新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐830091

出　　处：《新疆农业科学》2023年第12期3065-3071,共7页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区自然科学基金项目(2019D01A61);农业科技创新稳定支持项目(xjnkywdzc-2023005-6)。

摘　　要：【目的】优化从新疆哈密七角井盐湖土壤中分离到一株四氢嘧啶产量较高的中度嗜盐菌N35-6发酵培养基。【方法】以四氢嘧啶产量为指标,采用响应面法优化培养基成分,利用HPLC检测四氢嘧啶产量。【结果】从七角井盐湖土壤中分离获得一株能够产四氢嘧啶的细菌,经16S rDNA序列分析鉴定为Halomonas salifodinae,并命名为H.salifodinae N35-6,该菌株四氢嘧啶产量能够达到0.83 g/L。【结论】中度嗜盐菌Halomonas salifodinae N35-6具有高产四氢嘧啶的潜力。【Objective】A moderately halophilic bacterium Halomonas salifodinae N35-6 with high ectoine production was isolated from the soil of Qijaojing salt lake in Hami,Xinjiang,and its fermentation medium was optimized.【Methods】The medium composition for producing ectoine was optimized using response surface methodology and the yield of ectoine was determined by HPLC.【Results】A bacterium capable of producing ectoine was isolated from the soil of Qijiaojing Salt Lake and identified as Halomonas salifodinae by 16S rDNA sequence analysis.Production of ectoine was maximized to 0.83 g/L by optimizing the level of nutrients in the medium using response surface methodology.【Conclusion】The moderately halophilic bacterium Halomonas salifodinae N35-6 has the potential of high yield of ectoine.

关 键 词：中度嗜盐菌 四氢嘧啶 优化 

分 类 号：S188[农业科学—农业基础科学]