LncRNA XIST和miR-101-3p在SLE患者外周血单核细胞中的表达及临床应用价值  

Expression and clinical value of long non-coding RNA XIST and miR-101-3p in peripheral blood mononuclear cells of pa⁃tients with systemic lupus erythematosus

作　　者：赵琳[1] 倪成华 李洁[1] 梁波[3] 付冬冬[1] ZHAO Lin;NI Chenghua;LI Jie;LIANG Bo;FU Dongdong(Department of Rheumatology and Immunization,Xinxiang Central Hospital,Xinxiang 453000,China;Department of Acupuncture and Moxibustion,the Fourth People's Hospital of Xinxiang City,Xinxiang 453000,China;Department of Hematology,Xinxiang Central Hospital,Xinxiang 453000,China)

机构地区：[1]新乡市中心医院风湿免疫科,河南新乡453000 [2]新乡市第四人民医院针灸科,河南新乡453000 [3]新乡市中心医院血液科,河南新乡453000

出　　处：《安徽医学》2024年第1期28-33,共6页Anhui Medical Journal

基　　金：河南省医学科技攻关计划项目(编号:LHGJ20191329)。

摘　　要：目的分析长链非编码RNA(Lnc RNA)X染色体失活特异转录本(XIST)和微小RNA-101-3p(mi R-101-3p)在系统性红斑狼疮(SLE)患者外周血单核细胞中的表达及临床应用价值。方法选取2019年4月至2021年10月在新乡市中心医院治疗的180例SLE患者作为观察组,选择同期180例健康体检者作为对照组。根据SLE疾病活动指数(SLEDAI)评分将观察组分为稳定期组80例和活动期组100例。采用实时荧光定量聚合酶链反应(q RT-PCR)法对患者外周血单核细胞中Lnc RNA XIST、mi R-101-3p的相对表达水平进行检测。分析Lnc RNA XIST与mi R-101-3p的相关性以及二者与SLEDAI评分的相关性;对影响SLE的因素进行logistic回归分析;采用受试者工作特征(ROC)曲线分析Lnc RNA XIST、mi R-101-3p的表达对SLE的诊断价值。结果与对照组相比,观察组患者外周血单核细胞Lnc RNA XIST水平升高,mi R-101-3p水平降低,差异均有统计学意义(P<0.05);活动期组患者较稳定期组外周血单核细胞中Lnc RNA XIST水平升高,mi R-101-3p水平降低,差异均有统计学意义(P<0.05);患者外周血单核细胞Lnc RNA XIST与mi R-101-3p水平呈负相关(r=-0.410,P<0.05);Lnc RNA XIST水平与SLEDAI评分呈正相关(r=0.425,P<0.05),mi R-101-3p水平与SLEDAI评分呈负相关(r=-0.454,P<0.05);logistic回归分析显示,Lnc RNA XIST是影响SLE的危险因素(P<0.05),mi R-101-3p是影响SLE的保护因素(P<0.05);Lnc RNA XIST、mi R-101-3p联合诊断SLE的ROC曲线下面积为0.960,均优于其各自单独诊断(Z_(二者联合-Ln-c RNA XIST)=3.268,P=0.001;Z_(二者联合-mi R-101-3p)=2.584,P=0.005)。结论SLE患者外周血单核细胞Lnc RNA XIST水平升高,mi R-101-3p水平降低,与SLE疾病活动性有关,对SLE具有一定的诊断价值。Objective To analyze the expression of long non-coding RNA(LncRNA)X chromosome inactivation specific transcript(XIST)and microRNA-101-3p(miR-101-3p)in peripheral blood mononuclear cells of patients with systemic lupus erythematosus and their clinical application value.Methods A total of 180 patients with systemic lupus erythematosus who were treated in Xinxiang Central Hospital from April 2019 to October 2021 were selected as the observation group,and 180 healthy people in the same period were recruited as the control group.According to SLEDAI score,the patients were divided into stable stage group(80 cases)and active stage group(100 cases).The relative expression levels of LncRNA XIST and miR-101-3p in peripheral blood mononuclear cells of patients were detected by real-time fluorescent quantitative PCR(qRT-PCR).The correlation of LncRNA XIST with miR-101-3p and correlation of the two with SLEDAI scores were analyzed,logistic regression analysis was carried out on the factors influencing SLE,receiver operating characteristic curve(ROC)was used to analyze the diagnostic value of the expression of LncRNA XIST and miR-101-3p for systemic lupus erythematosus.Results Compared with the control group,the level of LncRNA XIST in peripheral blood mononuclear cells of systemic lupus erythematosus patients in the observation group was obviously higher,the level of miR-101-3p was obviously lower,and the differences were statistically significant(P<0.05);the level of LncRNA XIST in peripheral blood mononuclear cells of patients with systemic lupus erythematosus in the active phase group was markedly higher than that in the stable phase group,while the level of miR-101-3p was apparently lower,and the differences were statistically signifi-cant(P<0.05),there was a negative correlation between LncRNA XIST and miR-101-3p levels in peripheral blood monocytes of patients(r=-0.410,P<0.05),LncRNA XIST level was positively correlated with SLEDAI score(r=0.425,P<0.05),and miR-101-3p level was negatively correlated with SLEDAI score(r=

关 键 词：系统性红斑狼疮 长链非编码RNA X染色体失活特异转录本 微小RNA-101-3p 

分 类 号：R593.241[医药卫生—内科学]