PATL1对胃癌细胞增殖和迁移能力的影响及其作用机制  

Effects of PATL1 on the proliferation and migration of gastric cancer cells and its mechanism of action

作　　者：徐泽宇 莱智勇 任益凡 武锋 彭雨婷 徐钧[2] Xu Zeyu;Lai Zhiyong;Ren Yifan;Wu Feng;Peng Yuting;Xu Jun(Dept of Biochemistry and Molecular Biology,School of Basic Medicine,Shanxi Medical University,TaiYuan 030001;Dept of Hepatobiliary and Pancreatic Surgery,First Hospital of Shanxi Medical University,TaiYuan 030001)

机构地区：[1]山西医科大学基础医学院生物化学与分子生物学教研室,太原030001 [2]山西医科大学第一医院肝胆胰外科,太原030001

出　　处：《安徽医科大学学报》2024年第1期133-139,共7页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:82072737)。

摘　　要：目的探讨PAT家族蛋白1(PATL1)对胃癌细胞增殖和迁移的影响及其潜在的作用机制。方法通过TCGA数据库分析PATL1在泛癌、胃癌组织与正常组织中的表达水平;采用定量逆转录聚合酶链式反应(RT-qPCR)实验评估40例人胃癌组织及配对癌旁组织中PATL1的表达水平;通过Kaplan-Meier Plotter数据库分析PATL1对胃癌患者预后情况;在胃癌细胞株AGS中转染PATL1干扰载体,RT-qPCR实验评估干扰效果,采用细胞计数试剂盒8(CCK-8)、Transwell实验及划痕愈合实验检测PATL1对AGS增殖和迁移的影响;采用Western blot实验检测干扰PATL1后对胃癌细胞中细胞-髓细胞瘤病病毒致癌基因(c-Myc),自噬相关蛋白7(ATG7)蛋白表达的影响。结果RT-qPCR实验显示,PATL1在人胃癌组织中表达高于正常胃组织(P<0.001),并且PATL1与肠型胃癌患者预后有相关性(P<0.0001)。敲低PATL1后,增殖、迁移的胃癌细胞数目减少(P<0.05)。Western blot实验检测结果显示敲低PATL1后c-Myc、ATG7蛋白表达水平也随之下降(P<0.05)。结论PATL1可能通过与c-Myc、ATG7相互串扰抑制了胃癌细胞的增殖和迁移。Objective To explore the effect of PAT family proteins homolog 1(PATL1)on the proliferation and migration of gastric cancer cells and its potential mechanism.Methods The expression levels of PATL1 in pancarcinoma,gastric cancer and normal tissues were analyzed by TCGA database.The expression level of PATL1 in 40 human gastric cancer tissues and paired adjacent tissues was evaluated by quantitative real-time PCR(RT-qPCR).The Kaplan-Meier Plotter database was used to analyze the prognosis of PATL1 in gastric cancer patients.The gastric cancer cell line AGS was transfected with PATL1 interference vector,and the interference effect was evaluated by RT-qPCR.The effects of PATL1 on the proliferation and migration of AGS were detected by cell counting kit-8(CCK-8),Transwell test and scratch healing test.The effects of interference with PATL1 on the expression of cellular-myelocytomatosis viral oncogene(c-Myc)and autophagy related 7(ATG7)proteins in gastric cancer cells were detected by Western blot assay.Results RT-qPCR showed that the expression of PATL1 in human gastric cancer tissue was higher than that in normal gastric tissue(P<0.001),and PATL1 was correlated with the prognosis of patients with enteric gastric cancer(P<0.0001).After PATL1 was knocked down,the number of proliferating and migrating gastric cancer cells decreased(P<0.05).Western blot test results showed that the expression level of ATG7 protein decreased after PATL1 was knocked down(P<0.05).Conclusion PATL1 may inhibit the proliferation and migration of gastric cancer cells through crosstalk with c-Myc and ATG7.

关 键 词：胃癌 增殖 迁移 PATL1 自噬相关蛋白 

分 类 号：R34[医药卫生—基础医学]