Effects of pyraclostrobin on growth,oxidative stress,and gene expression in relation to stress and ATP-binding cassette transporters in Tetrahymena thermophila  

作　　者：Yang LIU Jiale ZHANG Peng XIAO Xin LIU Yisifu MA Jing ZHANG Bangjun ZHANG 

机构地区：[1]Henan International Joint Laboratory of Aquatic Ecotoxicology and Health Protection,Xinxiang 453007,China [2]College of Life Sciences,Henan Normal University,Xinxiang 453007,China [3]Journal of Henan Normal University,Xinxiang 453007,China [4]College of Life and Environmental Science,Wenzhou University,Wenzhou 325035,China [5]Department of Ecosystem Studies,School of Environmental Science,University of Shiga Prefecture,Shiga 522-8533,Japan

出　　处：《Journal of Oceanology and Limnology》2024年第1期150-162,共13页海洋湖沼学报（英文）

基　　金：the Key Scientific Research Projects of Henan Province to College Youth Backbone Teacher(No.2021118);the National Key Research and Development Program of China(No.2021YFE0112000)。

摘　　要：Pyraclostrobin(PYR),a widely used fungicide,has negative effects on fish and algae,but its toxicity in protozoa remains unclear.In this study,the effects of PYR on the growth,oxidative stress,and gene expression related to stress and ATP-binding cassette(ABC)transporters in Tetrahymena thermophila were investigated.The result showed that the 96-h IC_(50)of PYR against T.thermophila was 17.2 mg/L.Moreover,PYR inhibited the growth of T.thermophila in concentration-or time-dependent manner.A morphological study revealed that the shape and size of T.thermophila changed,and damage of cell membrane surface was observed by scanning electron microscopy after 96 h of PYR exposure.The activities of superoxide dismutase(SOD)and catalase(CAT)increased throughout the experiment.In contrast,the glutathione(GSH)content was increased at 24 h and 48 h of exposure and decreased at 96 h.Moreover,a significant increase in malondialdehyde(MDA)level was observed in T.thermophila after96 h of exposure.Furthermore,PYR upregulated the HSP703,HSP705,GPx2,and ABAC15 gene expression in the 0.1–5-mg/L groups and downregulated the HSP704,HSP90,TGR,and ABCC52 mRNA levels at 96 h of exposure.These results suggest that PYR may exert adverse effects on T.thermophila by inducing oxidative stress and changing the gene expression related to ABC transporters and stress,which may enrich the understanding of the toxicity mechanism of PYR in aquatic organisms and provide reference data for aquatic ecological risk assessments.

关 键 词：PYRACLOSTROBIN Tetrahymena thermophila GROWTH oxidative stress gene expression 

分 类 号：X592[环境科学与工程—环境工程] X171.5