Phlorizin alleviates deltamethrin-induced oxidative stress in brine shrimp Artemia  

作　　者：Dandan MA Qingli ZHOU Liying SUI Qingbin GUO Huanhuan LIU Honghe LIANG Zhenjing LI Zhongna SANG 

机构地区：[1]School of Food Science and Engineering,Tianjin University of Science and Technology,Tianjin 300457,China [2]Asian Regional Artemia Reference Center,Key Laboratory of Marine Resource Chemistry and Food Technology(TUST),Ministry of Education,Tianjin University of Science and Technology,Tianjin 300457,China [3]Subtropical Crops Research Institute of Guangxi Zhuang Autonomous Region,Nanning 530001,China [4]Department of Nutrition and Food Hygiene,School of Public Health,Tianjin Medical University,Tianjin 300070,China

出　　处：《Journal of Oceanology and Limnology》2024年第1期163-173,共11页海洋湖沼学报（英文）

基　　金：the Key Laboratory of Marine Resource Chemistry and Food Technology(TUST);the Ministry of Education(No.EMTUST-21-08);the Guilin Science and Technology Project(No.20210225-4)。

摘　　要：Deltamethrin(DEL),a commonly used pyrethroid pesticide,results in higher reactive oxygen species(ROS)levels in aquatic animals,which consequently unbalance the redox state.Phlorizin(PHL)is a flavonoid and a natural product promising to prevent or reduce pesticide-induced oxidative stress.Artemia is a micro-crustacean widely used in marine hatcheries and an experimental aquatic organism for environmental toxicology research.This research aimed to evaluate the toxicity of DEL on Artemia and the antioxidative effect of PHL against the toxicity.Results show that 0.08-mg/mL PHL exerted its antioxidative effects on hatching percentage of the cysts in 24-h incubation and on body length and survival rate of Artemia in 12-d culture.After 12-d culture,12-,24-,and 36-h DEL exposure showed significant drops in SOD,CAT,and GSH-Px enzyme activities,and significant increases in ROS and malondialdehyde(MDA)levels in Artemia(P<0.05).On the contrary,0.08-mg/mL PHL application improved the enzyme activities and decreased the ROS and MDA levels(P<0.05).Moreover,0.08-mg/mL PHL significantly increased mRNA expression levels of Cu/Zn SOD,CAT,GST,HO-1,NQO1,and Nrf2,and decreased mRNA expression level of Keap1 in the DEL-exposed Artemia(P<0.05).Therefore,DEL is toxic to Artemia,while PHL alleviates DEL-induced oxidative damage by possibly regulating the Nrf2signaling pathway.This study provided a theoretical basis for PHL to reduce pesticide-induced toxicity in aquatic animals.

关 键 词：ARTEMIA DELTAMETHRIN PHLORIZIN oxidative stress 

分 类 号：X592[环境科学与工程—环境工程]