Transcriptome analysis reveals immune-related genes in tissues of Vibrio anguillarum-infected turbot Scophthalmus maximus  

作　　者：Yuting SONG Maqsood Ahmed SOOMRO Xianzhi DONG Guobin HU 

机构地区：[1]College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China [2]Institute of Evolution&Marine Biodiversity,Ocean University of China,Qingdao 266003,China [3]Institute of Biophysis,Chinese Academy of Sciences,Beijing 100101,China

出　　处：《Journal of Oceanology and Limnology》2024年第1期332-344,共13页海洋湖沼学报（英文）

基　　金：the National Key Research and Development Program of the Ministry of Science and Technology(CN)(No.2022YFD2400401);the Key Research and Development Plan of Shandong Province(CN)(for Academician Team in Shandong)(No.2023ZLYS02);the Fundamental Research Funds for the Central Universities(No.202261029);the Enterprise Authorized Project(No.20200025)。

摘　　要：Turbot Scophthalmus maximus is an important mariculture fish species with high economic value.However,the bacterial diseases caused by Vibrio anguillarum infection bring huge economic losses to the turbot aquaculture industry.To understand the immune response of the turbot against V.anguillarum infection and to explore novel immune-related genes,the transcriptome analysis of turbot spleen and gills were conducted after V.anguillarum infection.Differentially expressed genes(DEGs)were identified in spleen and gill of the turbot amounted to 17261 and 16436,respectively.A large number of immunerelated DEGs were enriched in cytokine-cytokine receptor interaction signaling pathway,and the others by the kyoto encyclopedia of genes and genomes(KEGG)enrichment.The gene ontology(GO)classification analysis revealed that V.anguillarum infection had the greatest effect on biological processes and cellular components.Twelve immune-related DEGs were identified in the spleen(cstl.1,egfl6,lamb21,v2rx4,calcr,and gpr78a)and gills(ghra,sh3gl2a,cst12,inhbaa,cxcl8,and il-1b)by heat map.The proteinprotein interaction(PPI)networks were constructed to analyze the immune mechanism.The results demonstrate that the maturation and antigen processing of major histocompatibility complex(MHC)class II molecule,and calcitonin-or adrenomedullin-regulated physiological activity were important events in the immunity of turbot against V.anguillarum infection.In the gills,the protein interactions in TGF-βsignaling pathway,production of inflammatory factors,and endocytosis regulation were most significant.Our research laid a foundation for discovering novel immune-related genes and enriching the knowledge of immune mechanisms of turbot against V.anguillarum infection.

关 键 词：Scophthalmus maximus Vibrio anguillarum TRANSCRIPTOME differentially expressed genes immune mechanism 

分 类 号：S943[农业科学—水产养殖]