深海来源帚状弯孢聚壳菌(Eutypella scoparia W2)的化学成分研究  

Chemical constituents of deep-sea-derived Eutypella scoparia W2

作　　者：胡曼宜 蒋家阳 徐琳 刘柱[2] 杨献文 HU Man-yi;JIANG Jia-yang;XU Lin;LIU Zhu;YANG Xian-wen(Key Laboratory of Marine Genetic Resources,Third Institute of Oceanography,Ministry of Natural Resources,Xiamen Fujian 361005;College of Life Sciences,Hainan University,Haikou 570228)

机构地区：[1]自然资源部第三海洋研究所海洋生物遗传资源重点实验室,福建厦门361005 [2]海南大学生命科学学院,海口570228

出　　处：《中南药学》2023年第12期3141-3144,共4页Central South Pharmacy

基　　金：国家自然科学基金项目(No.22177143)。

摘　　要：目的 研究深海来源帚状弯孢聚壳菌的化学成分。方法 采用硅胶柱色谱、葡聚糖凝胶色谱、高效液相色谱等现代色谱分离技术,对帚状弯孢聚壳菌的发酵粗提物进行系统的分离纯化,对所获得的化合物采用核磁等波谱数据分析,最终确定其化学结构,并对其进行抑菌活性测试。结果 从帚状弯孢聚壳菌中分离获得了6个天然化合物,分别鉴定为penicopeptide A(1)、viridicatin(2)、cyclopenol(3)、ergosterol peroxide(4)、pestalotiopin A(5)、4-methyl-5,6-dihydropyren-2-one(6)。6个化合物对4种致病菌均未显示出很强的抑制活性(在20μmol·L^(-1)浓度下,抑菌率低于50%)。结论 化合物1、2、3、5、6均为首次从该属真菌中分离得到。Objective To determine the chemical constituents of deep-sea-derived Eutypella scoparia W2.Methods The crude extract of Eutypella scoparia W2 was systematically isolated and purified by column chromatography on repeated silica gel,Sephadex LH-20,and ODS,etc.The chemical structures of the isolated compounds were identified by extensive analysis of the NMR spectroscopic data.The inhibitory activities of all isolates were investigated against 4 pathogenic bacteria.Results Totally 6 compounds were obtained from Eutypella scoparia W2,including penicopeptide A(1),viridicatin(2),cyclopenol(3),ergosterol peroxide(4),pestalotiopin A(5),and 4-methyl-5,6-dihydropyren-2-one(6).However,none showed significant antibacterial effects,namely growth inhibition rate lower than 50%under the concentration of 20μmol·L^(-1).Conclusion Compounds 1,2,3,5,and 6 have been isolated from the genus of Eutypella for the first time.

关 键 词：深海 真菌 帚状弯孢聚壳菌 化学成分 

分 类 号：R284.1[医药卫生—中药学]