不同叶色猴樟花青素含量与光合特性比较  

Comparison of Anthocyanin Content and Photosynthetic Characteristics of Cinnamomun bodinieri Seedlings with Different Leaf Colors

作　　者：张楠[1] 韩浩章[1] 张丽华[1] 李素华[1] 赵荣 王芳[1] Zhang Nan;Han Haozhang;Zhang Lihua;Li Suhua;Zhao Rong;Wang Fang(Institute of Biology and Materials,Suqian University,Suqian 223800,China)

机构地区：[1]宿迁学院生物与材料学院,江苏宿迁223800

出　　处：《中国野生植物资源》2024年第1期64-69,共6页Chinese Wild Plant Resources

基　　金：江苏省自然科学基金项目(BK20201481);宿迁市科技计划项目(L202004,M202001);宿迁学院重点建设学科项目(2021ZDJS04);宿迁学院创新团队项目(2021td05)。

摘　　要：目的:比较不同叶色猴樟花青素含量与光合特性差异,初步分析猴樟春季叶色形成的机理。方法:以3种不同叶色的猴樟种苗为材料,测定其功能叶片花青素含量、光合色素含量、叶绿素荧光参数、光合作用参数及花青素合成相关基因表达量。结果:紫叶猴樟和红叶猴樟的叶绿素a、叶绿素b、叶绿素(a+b)和类胡萝卜素含量均与绿叶猴樟无明显差异,花青素含量分别是绿叶猴樟的134.24倍和49.80倍;红叶猴樟和紫叶猴樟的净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)均高于绿叶猴樟,胞间CO_(2)浓度(Ci)和水分利用效率(WUE)均低于绿叶猴樟;红叶猴樟和紫叶猴樟的初始荧光(Fo)、暗适应下最大荧光(Fm)、非光化学淬灭系数(NPQ)、光适应下PSII的最大量子产额(Fo’/Fm’)均高于绿叶猴樟,光化学淬灭系数(qP)和光适应下PSII的实际光化学效率(ΦPSⅡ)均低于绿叶猴樟,暗适应下PSⅡ最大光化学效率(Fv/Fm)差异不显著;紫叶猴樟的CbDFR、CbANS和CbUFGT基因表达量显著高于绿叶猴樟,红叶猴樟的CbDFR、CbANS和CbUFGT基因表达量高于绿叶猴樟,其中仅有CbUFGT基因表达量差异显著。结论:花青素是猴樟叶片呈紫、红色的主要色素,花青素对猴樟叶片光合系统具有保护功能;CbDFR、CbANS和CbUFGT可能是猴樟叶片呈色的关键基因。Objective:The differences in anthocyanin content and photosynthetic characteristics of different leaf colors of Cinnamomun bodinieri were compared,and the mechanism of leaf color formation in spring was analyzed preliminarily.Methods:The leaf anthocyanin content,chlorophyll content,chlorophyll fluorescence parameters,photosynthetic parameters and the expression of anthocyanin synthesis-related genes were determined in three C.bodinieri seedlings with different leaf colors.Results:The content of chlorophyll a,chlorophyll b,chlorophyll(a+b),and carotenoid in purple and red leaf seedlings was not significantly different from that in green seedlings,but the content of anthocyanin in leaves of purple and red seedlings was 134.24 and 49.80 times higher than that of green seedlings,respectively.The net photosynthetic rate(Pn),stomatal conductance(Gs)and transpiration rate(Tr)of red and purple leaf seedlings were higher than those of green leaf seedlings,while the intercellular CO_(2) concentration(Ci)and water use efficiency(WUE)were lower than those of green leaf seedlings.In terms of photosystem function,the initial fluorescence(Fo),the maximum fluorescence under dark adaptation(Fm),the nonphotochemical quenching coefficient(NPQ),and the maximum quantum yield of PSII under light adaptation(Fo'/Fm')of red and purple leaf seedlings were higher than those of green leaf seedlings,but the photochemical quenching coefficient(qP)and the actual photochemical efficiency(ΦPSⅡ)were lower than those of green leaf seedlings,and the significant difference was not founded about the maximum photochemical efficiency of PSII under dark adaptation(Fv/Fm)in three C.bodinieri seedlings.The CbDFR,CbANS and CbUFGT gene expression levels of the purple leaf seedlings were significantly higher than those of the green leaf seedlings.The CbDFR,CbANS and CbUFGT gene expression levels of the red leaf seedlings were higher than those of the green leaf seedlings,and only CbUFGT was significantly different.Conclusion:Anthocyanin was regarded as th

关 键 词：猴樟 花青素合成 光合作用 叶绿素荧光 

分 类 号：S792.2[农业科学—林木遗传育种]