中药复方参麻颈复颗粒治疗脑梗死的网络药理学研究  

Study on the treatment of cerebral infarction with Chinese medicine Shenmajingfu granule by network pharmacology

作　　者：徐熠[1] 何瑞华 黄瑾 XU Yi;HE Ruihua;HUANG Jin(Department of Pharmacy,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China)

机构地区：[1]上海中医药大学附属岳阳中西医结合医院药剂科,上海200437

出　　处：《药学实践与服务》2024年第2期66-72,共7页Journal of Pharmaceutical Practice and Service

基　　金：上海市进一步加快中医药传承创新发展三年行动计划[ZY(2021-2023)-0203-03];上海中医药大学附属岳阳中西医结合医院科研项目(2019YYZ07);上海申康医院发展中心临床科技创新项目(SHDC12021635);中国药学会全国医药信息网2022年度科普研究重点项目(CMEI2022KPYJ00296);上海中医药大学第二十一期课程建设项目(No.2022SHUTCM 147)。

摘　　要：目的应用网络药理学的研究方法探索中药复方参麻颈复颗粒治疗脑梗死的物质基础及其作用机制。方法通过TCMSP数据库、ETCM数据库、中医药资料库检索参麻颈复颗粒中潜在活性成分及作用靶点;OMIM数据库检索脑梗死相关靶蛋白基因,采用交集法获得参麻颈复颗粒与脑梗死的共同靶点基因。运用Cytoscape构建“参麻颈复活性成分-靶点”网络,利用STRING数据库构建PPI网络,采用DAVID数据库进行GO和KEGG富集分析。结果筛选出参麻颈复颗粒的183个潜在有效成分,在TCMSP数据库中筛选到1785个潜在靶点蛋白,其中与脑梗死有关的作用靶点30个,这些靶点基因主要参与炎症反应和细胞凋亡过程,涉及TNF信号通路、HIF-1信号通路、NF-κB信号通路等。结论参麻颈复颗粒对脑梗死的治疗作用可能与调控炎症反应、改善受损神经功能、保护脑缺血再灌注损伤有关。Objective To explore the material basis and mechanism of the Chinese medicine Shenmajingfu granules in the treatment of cerebral infarction.Methods The potential active ingredients and targets of Shenmajingfu granules were retrieved through TCMSP,ETCM database and TCM Database.The related target genes of cerebral infarction were searched from OMIM database.The common targets of Shenmajingfu granules and cerebral infarction were obtained by the intersection method.Cytoscape was used to construct active components of Shenmajingfu granules-targets network.Protein-protein interaction network was constructed by STRING software.DAVID database was used for GO and KEGG enrichment analysis.Results The 183 potential active ingredients of Shenmajingfu granules were screened out.1785 potential targets were screened in the TCMSP database,including 30 targets related to cerebral infarction.These target genes were mainly involved in the inflammatory response and apoptosis process,involving the TNF signaling pathway,HIF-1 signaling pathway and NF-κB signaling pathway.Conclusion The therapeutic effect of Shenmajingfu granules on cerebral infarction may be related to the regulation of inflammatory response,improvement of impaired neurological function and protection of cerebral ischemia-reperfusion injury.

关 键 词：参麻颈复颗粒 脑梗死 网络药理学 

分 类 号：R285[医药卫生—中药学]