支气管哮喘患儿外周血suPAR、JAK/STAT信号通路与气道重构的相关性分析  

Correlation analysis of peripheral blood suPAR,JAK/STAT signaling pathways and airway remodeling in children with bronchial asthma

作　　者：屈乐言 罗建江[1] 张多兰 郭欢 Qu Leyan;Luo Jianjiang;Zhang Dolan;Guo Huan(Department of Infectious Diseases,The Fourth Affiliated Hospital of Xinjiang Medical University,Xinjiang Province,Urumqi 830000,China;不详)

机构地区：[1]新疆医科大学第四附属医院感染性疾病科,乌鲁木齐830000 [2]新疆医科大学第四附属医院呼吸重症监护室,乌鲁木齐830000 [3]吐鲁番市高昌区人民医院呼吸与危重症医学科,838000

出　　处：《疑难病杂志》2024年第2期160-164,共5页Chinese Journal of Difficult and Complicated Cases

基　　金：国家自然科学基金资助项目(82060794);新疆维吾尔自治区自然科学基金资助项目(2022D01E80);天山青年计划项目(2020Q048)。

摘　　要：目的探究支气管哮喘(BA)患儿外周血可溶性尿激酶型纤溶酶原激活物受体(suPAR)、酪氨酸激酶/信号传导及转录激活因子(JAK/STAT)信号通路与气道重构的相关性。方法选取2021年1月—2023年1月吐鲁番市高昌区人民医院呼吸与危重症医学科收治的BA患儿106例为研究对象(BA组),根据哮喘分级标准分为间歇状态亚组(n=58)、轻度亚组(n=29)和中重度亚组(n=19)。另选取同期健康体检儿童106例为健康对照组。酶联免疫吸附法(ELISA)测定血清suPAR水平,实时荧光定量PCR方法测定血清JAK、STAT水平。比较不同组间患儿血清suPAR、JAK、STAT水平、肺功能及气道重构状况;分析血清suPAR水平、JAK/STAT与BA患儿肺功能及气道重构的关系。结果与健康对照组比较,BA组患儿血清suPAR、JAK、STAT水平及气道壁厚度与气道腔外径比(T/D)、气道壁总面积占气道总面积百分比(WA%)均显著升高(t/P=20.572/<0.001,16.640/<0.001,16.182/<0.001,14.414/<0.001,19.359/<0.001),第1秒用力呼气容积(FEV_(1))占预计值百分比(FEV_(1)%)、FEV_(1)占用力肺活量百分比(FEV_(1)/FVC)均降低(t/P=22.796/<0.001,15.559/<0.001);间歇状态亚组、轻度亚组、中重度亚组BA患儿中,血清suPAR、JAK、STAT水平及T/D、WA%依次升高(F/P=23.667/<0.001,52.475/<0.001,30.306/<0.001,76.897/<0.001,62.594/<0.001),FEV_(1)%、FEV_(1)/FVC依次降低(F/P=99.545/<0.001,91.936/<0.001);Pearson相关性分析显示,血清suPAR、JAK、STAT水平与FEV_(1)%、FEV_(1)/FVC均呈显著负相关(suPAR:r/P=-0.467/<0.001,-0.424/<0.001,JAK:r/P=-0.601/<0.001,-0.560/<0.001,STAT:r/P=-0.458/<0.001,-0.412/<0.001),与T/D、WA%均呈显著正相关(suPAR:r/P=0.427/<0.001,0.411/<0.001,JAK:r/P=0.541/<0.001,0.455/<0.001,STAT:r/P=0.477/<0.001,0.484/<0.001),且FEV_(1)%、FEV_(1)/FVC与T/D、WA%呈显著负相关(T/D:r/P=-0.627/<0.001,-0.546/<0.001,WA%:r/P=-0.590/<0.001,-0.504/<0.001)。结论suPAR高表达及JAK/STAT通路激活可能与BA的发生发展有关,且suPAR水平�Objective To investigate the correlation between peripheral blood soluble urokinase type plasminogen activator receptor(suPAR),tyrosine kinase/signaling and transcription activating factor(JAK/STAT)signaling pathways and airway remodeling in children with bronchial asthma(BA).Methods One hundred and six children with BA admitted to the Respiratory and Critical Care Medicine Department of Gaochang District People's Hospital in Turpan City from January 2021 to January 2023 were selected as the study subjects(BA group).According to the asthma grading criteria,they were divided into intermittent subgroups(n=58),mild subgroups(n=29),and moderate to severe subgroups(n=19).106 healthy children who underwent physical examinations during the same period were selected as the healthy control group.Enzyme linked immunosorbent assay(ELISA)was used to measure serum suPAR levels,while real-time fluorescence quantitative PCR was used to measure serum JAK and STAT levels.Compare the serum levels of suPAR,JAK,STAT,lung function,and airway remodeling among different groups of children;Analyze the relationship between serum suPAR levels,JAK/STAT,lung function,and airway remodeling in children with BA.Results Compared with the healthy control group,the serum levels of suPAR,JAK,STAT,the ratio of airway wall thickness to airway outer diameter(T/D),and the percentage of total airway wall area to total airway area(WA%)in the BA group were all increased(t/P=20.572/<0.001,16.640/<0.001,16.182/<0.001,14.414/<0.001,19.359/<0.001),and the percentage of forced expiratory volume(FEV_(1))to expected value at the first second(FEV_(1)%)The percentage of FEV_(1) to forced lung capacity(FEV_(1)/FVC)decreased(t/P=22.796/<0.001,15.559/<0.001);In the intermittent state subgroup,mild subgroup,and moderate to severe subgroup of BA patients,serum levels of suPAR,JAK,STAT,T/D,and WA%increased sequentially(F/P=23.667/<0.001,52.475/<0.001,30.306/<0.001,76.897/<0.001,62.594/<0.001),while FEV_(1)%,FEV_(1)/FVC decreased sequentially(F/P=99.545/<0.001,91.936/<0

关 键 词：支气管哮喘 气道重构 可溶性尿激酶型纤溶酶原激活物受体 酪氨酸激酶/信号传导及转录激活因子 相关性 

分 类 号：R562.2[医药卫生—呼吸系统]