cAMP/CREB信号通路对丙泊酚麻醉致大鼠认知功能损伤、记忆功能的影响及其机制  

The effects and mechanisms of the cAMP/CREB signaling pathway on cognitive and memory impairment induced by propofol anesthesia in rats

作　　者：吴帮林 张伟 朱荣誉 吴述轩 朱贤林 Wu Banglin;Zhang Wei;Zhu Rongyu;Wu Shuxuan;Zhu Xianlin(Department of Anesthesiology,the Central Hospital of Enshi Tujia and Miao Autonomous Prefecture,Hubei Province,Enshi 445000,China)

机构地区：[1]恩施土家族苗族自治州中心医院麻醉科,湖北恩施445000

出　　处：《疑难病杂志》2024年第2期234-239,共6页Chinese Journal of Difficult and Complicated Cases

基　　金：湖北省自然科学基金项目(2016CFB368)。

摘　　要：目的探究环腺苷酸/环磷酸腺苷反应元件结合蛋白(cAMP/CREB)信号通路对丙泊酚麻醉致大鼠认知功能损伤、记忆功能的影响及机制。方法2021年12月—2022年2月于湖北省恩施土家族苗族自治州中心医院动物实验室进行实验。将30只大鼠按随机数字表法分为对照组、丙泊酚组、8-溴-环腺苷酸(8-Br-cAMP)+丙泊酚组各10只,8-Br-cAMP+丙泊酚组大鼠注射信号通路激动剂8-Br-cAMP+丙泊酚,丙泊酚组注射丙泊酚,对照组注射0.9%氯化钠。麻醉1 d后观察大鼠认知记忆功能变化。结果与对照组比较,丙泊酚组大鼠逃避潜伏期时间延长,穿越平台次数下降(P均<0.01);与丙泊酚组比较,8-Br-cAMP+丙泊酚组大鼠逃避潜伏期时间缩短,穿越平台次数上升(P均<0.01)。与对照组比较,丙泊酚组理毛次数、跨格次数、站立次数下降,中央格停留时间延长(P均<0.01);与丙泊酚组比较,8-Br-cAMP+丙泊酚组理毛次数、跨格次数、站立次数上升,中央格停留时间缩短(P均<0.01)。与对照组比较,丙泊酚组MDA水平上升,SOD水平下降(P均<0.01);与丙泊酚组比较,8-Br-cAMP+丙泊酚组MDA水平下降,SOD水平上升(P均<0.01)。与对照组比较,丙泊酚组cAMP、CREB mRNA表达量均下降(P均<0.01);与丙泊酚组比较,8-Br-cAMP+丙泊酚组cAMP、CREBmRNA表达量均上升(P均<0.01)。与对照组比较,丙泊酚组cAMP、CREB蛋白相对表达量均下降(P均<0.01);与丙泊酚组比较,8-Br-cAMP+丙泊酚组cAMP、CREB蛋白相对表达量均上升(P均<0.01)。结论激活cAMP/CREB信号通路可缓解丙泊酚麻醉造成认知功能损伤及记忆功能下降,其机制可能与氧化应激反应受到调节有关。Objective To explore the effects and mechanisms of the cAMP/CREB signaling pathway on cognitive and memory impairment induced by propofol anesthesia in rats.Methods From December 2021 to February 2022,an experiment was conducted in the Animal Laboratory of Enshi Tujia and Miao Autonomous Prefecture Central Hospital in Hubei Province.Thirty rats were randomly divided into a control group,a propofol group,and a group of 10 rats each containing 8-Br-cAMP and propofol.The 8-Br-cAMP+propofol group was injected with the signal pathway agonist 8-Br-cAMP+propofol,while the propofol group was injected with propofol and the control group was injected with 0.9%sodium chloride.Observe the changes in cognitive and memory function of rats after 1 day of anesthesia.Results Compared with the control group,the propofol group rats had a longer escape latency time and a lower number of crossing platforms(P<0.01 for all);Compared with the propofol group,the rats in the 8-Br-cAMP+propofol group had a shorter escape latency time and an increase in the number of crossing platforms(P<0.01 for all).Compared with the control group,the propofol group had a decrease in the number of grooming sessions,cross grid sessions,and standing sessions,while the central grid stay time was prolonged(P<0.01 for all);Compared with the propofol group,the 8-Br-cAMP+propofol group showed an increase in grooming frequency,cross grid frequency,and standing frequency,while the central grid residence time was shortened(P<0.01).Compared with the control group,the MDA level in the propofol group increased while the SOD level decreased(P<0.01 for all);Compared with the propofol group,the 8-Br-cAMP+propofol group showed a decrease in MDA levels and an increase in SOD levels(all P<0.01).Compared with the control group,the mRNA expression levels of cAMP and CREB in the propofol group decreased(P<0.01);Compared with the propofol group,the expression levels of cAMP and CREB mRNA in the 8-Br-cAMP+propofol group increased(P<0.01).Compared with the control group,the relat

关 键 词：丙泊酚 环腺苷酸/环磷酸腺苷反应元件结合蛋白 认知功能 记忆功能 氧化应激 大鼠 

分 类 号：R614[医药卫生—麻醉学]