In vivo polydopamine coating of Rhodobacter sphaeroides for enhanced electron transfer  

作　　者：Rossella Labarile Danilo Vona Maria Varsalona Matteo Grattieri Melania Reggente Roberto Comparelli Gianluca M.Farinola Fabian Fischer Ardemis A.Boghossian Massimo Trotta 

机构地区：[1]Istituto per i Processi Chimico Fisici,Consiglio Nazionale delle Ricerche,Bari 70126,Italy [2]Dipartimento di Chimica,Universitàdegli Studi di Bari,Bari 70126,Italy [3]Institute of Chemical Sciences and Engineering(ISIC),Ecole Polytechnique Fédérale de Lausanne(EPFL),Lausanne 1015,Switzerland [4]Institute of Life Technologies,HES-SO Valais-Wallis,1950 Sion,Switzerland [5]Institute for Renewable Energy,HES-SO Valais-Wallis,1950 Sion,Switzerland

出　　处：《Nano Research》2024年第2期875-881,共7页纳米研究（英文版）

基　　金：funded by the Fonds National Suisse de la Recherche Scientifique,project Phosbury-Photosynthetic bacteria in Self-assembled Biocompatible coatings for the transduction of energy(Project Nr CRSII5_205925/1);M.G.acknowledges the funding from Fondazione CON IL SUD,Grant“Brains to South 2018”(project number 2018-PDR-00914).

摘　　要：Recent advances in coupling light-harvesting microorganisms with electronic components have led to a new generation of biohybrid devices based on microbial photocatalysts.These devices are limited by the poorly conductive interface between phototrophs and synthetic materials that inhibit charge transfer.This study focuses on overcoming this bottleneck through the metabolically-driven encapsulation of photosynthetic cells with a bio-inspired conductive polymer.Cells of the purple non sulfur bacterium Rhodobacter sphaeroides were coated with a polydopamine(PDA)nanoparticle layer via the self-polymerization of dopamine under anaerobic conditions.The treated cells show preserved light absorption of the photosynthetic pigments in the presence of dopamine concentrations ranging between 0.05–3.5 mM.The thickness and nanoparticle formation of the membrane-associated PDA matrix were further shown to vary with the dopamine concentrations in this range.Compared to uncoated cells,the encapsulated cells show up to a 20-fold enhancement in transient photocurrent measurements under mediatorless conditions.The biologically synthesized PDA can thus act as a matrix for electronically coupling the light-harvesting metabolisms of cells with conductive surfaces.

关 键 词：BIOELECTRONICS photosynthetic bacteria purple bacteria electron transfer POLYDOPAMINE biophotovoltaics 

分 类 号：R318[医药卫生—生物医学工程]