三宝护童羹对变应性鼻炎幼鼠模型细胞免疫机制的影响研究  

Effect of Sanbao Hutong Decoction on Cellular Immune Mechanism of Allergic Rhinitis In Young Rat Model

作　　者：阳成琳 陈利 冯乔芳 冉志玲 YANG Cheng-lin;CHEN Li;FENG Qiao-fang;RAN Zhi-ling(Southwest Medical University,Luzhou 646000,Sichuan,China;Changning Hospital of Traditional Chinese Medicine,Changning 644300,China;The Affiliated Hospital of Traditional Chinese Medicine,Southwest Medical University,Luzhou 646000,China)

机构地区：[1]西南医科大学,四川泸州646000 [2]长宁县中医院,四川长宁644300 [3]西南医科大学附属中医医院,四川泸州646000

出　　处：《云南中医中药杂志》2024年第1期85-92,共8页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：四川省中医药管理局课题(2023MS464)。

摘　　要：目的通过免疫因子及蛋白表达水平变化探讨三宝护童羹制剂(SBHTG)对卵蛋白(OVA)致SD幼鼠过敏性鼻炎(AR)肺脾气虚型模型的治疗作用及机制研究。方法将32只2-3周龄SPF级SD大鼠分为A组:空白组,B组:模型组,C组:西药组,D组:中药组,每组各8只。适应性喂养5d后,除A组给予生理盐水模拟造模外,其余各组给予全身致敏+局部致敏法共计21d,自第15d起开始给药物干预,14d后处理。通过HE染色法评价鼻黏膜的病理形态学,以ELISA检测鼻炎相关细胞因子水平变化,取脾脏运用Western blot法检测炎症相关蛋白水平变化。结果与A组比较,B组幼鼠鼻部症状评分、血清IL-4及IgE含量显著升高(P<0.01),三宝护童羹制剂及氯雷他定干预后显著回调;血清中INF-γ含量及脾脏中Foxp3蛋白表达显著降低(P<0.01),三宝护童羹制剂及氯雷他定干预后显著上升。C、D组幼鼠鼻部症状评分、血清Ig E及IL-4水平下降明显(P<0.01);C、D组幼鼠血清INF-γ含量显著降低(P<0.01);C、D组脾脏组织中Foxp3蛋白表达显著升高(P<0.01);C、D组鼻黏膜上皮纤毛较模型组整齐连续,上皮水肿情况、杯状细胞及EOS浸润较B组减轻。结论推断三宝护童羹可有效调控变应性鼻炎模型幼鼠免疫功能,这可能与上调INF-γ水平及Foxp3蛋白表达水平,下调IL-4、IgE水平有关。Objective:To study the therapeutic effect and mechanism of Sanbao Hutong Decoction(SBHTG)on allergic rhinitis(AR)model of qi deficiency of lung and spleen in SD young mice induced by OVA through the changes of immune factors and protein expression levels.Methods:32 SPF SD rats aged 2-3 weeks were divided into blank group A,model group B,western medicine group C and Chinese medicine group D,8 rats each group.After 5 days of adaptive feeding,except for group A,the other groups were given systemic sensitization+local sensitization method for 21 days,drug intervention began on the 15th day,and the treatment was given 14 days later.The pathological morphology of nasal mucosa was evaluated by HE staining,the changes of rhinitis related cytokines were detected by ELISA,and the changes of inflammatory related proteins were detected by Western blot.Results:Compared with those of group A,the nasal symptom score,serum IL-4 and IgE contents were significantly increased in group B(P<0.01),and significantly decreased after intervention with Sanbao Hutong Decoction and loratadine.INF-γcontent in serum and Foxp3 protein expression in spleen were significantly decreased(P<0.01),and were significantly increased after intervention with Sanbao Hutong Decoction and loratadine.Nasal symptom scores,serum IgE and IL-4 levels of the rats in groups C and D were significantly decreased(P<0.01).Serum INF-γcontent in groups C and D was significantly decreased(P<0.01).The expression of Foxp3 protein in spleen tissues of groups C and D was significantly increased(P<0.01).The nasal mucosal cilia in groups C and D were more orderly and continuous than those in the model group,and the epithelial edema,goblet cells and EOS infiltration were less severe than those in group B.Conclusion:It is concluded that Sanbao Hutong Decoction can effectively regulate the immune function of young mice with allergic rhinitis model,which may be related to up-regulating INF-γlevel and Foxp3 protein expression level,and down-regulating IL-4 and IgE level.

关 键 词：变应性鼻炎 三宝护童羹 Foxp3蛋白 Γ干扰素 白细胞介素4 免疫球蛋白E 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]